Chinese Journal of Pharmaceuticals, Volume. 56, Issue 7, 877(2025)

Design, Preparation and Enzymatic Properties of Urate Oxidase with High Mucin Binding Activity

LI Zhigang1,2, WANG Yapeng2,3, LU Jianguang4, HUA Haoju4, and FENG Jun2,4、*
Author Affiliations
  • 1Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014
  • 2China State Institute of Pharmaceutical Industry, Shanghai 201203
  • 3School of Pharmacy, Fudan University, Shanghai 201203
  • 4Shanghai Duomirui Bio-Technology Co., Ltd., Shanghai 201203
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    References(14)

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    [8] [8] TANG Y K, DU Y W, YE J N,et al. Intestine-targeted explosive hydrogel microsphere promotes uric acid excretion for gout therapy [J].Adv Mater, 2024,36(3): e2310492.

    [9] [9] ZHAO R, LI Z M, SUN Y Q,et al. EngineeredEscherichia coliNissle 1917 with urate oxidase and an oxygen-recycling system for hyperuricemia treatment [J].Gut Microbes, 2022,14(1): 2070391.

    [10] [10] MACKENZIE D A, TAILFORD L E, HEMMINGS A M,et al. Crystal structure of a mucus-binding protein repeat reveals an unexpected functional immunoglobulin binding activity[J].J Biol Chem, 2009,284(47): 32444-32453.

    [11] [11] ETZOLD S, KOBER O I, MACKENZIE D A,et al. Structural basis for adaptation of lactobacilli to gastrointestinal mucus [J].Environ Microbiol, 2014,16(3): 888-903.

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    [14] [14] WANG Y, LU J, HUANG Z,et al. Process development of recombinantAspergillus flavusurate oxidase production inPichia pastorisintracellularly and its characterization as a potential biosimilar [J].Process Biochem, 2021,102: 376-385.

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    LI Zhigang, WANG Yapeng, LU Jianguang, HUA Haoju, FENG Jun. Design, Preparation and Enzymatic Properties of Urate Oxidase with High Mucin Binding Activity[J]. Chinese Journal of Pharmaceuticals, 2025, 56(7): 877

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    Paper Information

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    Received: Apr. 25, 2025

    Accepted: Aug. 26, 2025

    Published Online: Aug. 26, 2025

    The Author Email: FENG Jun (fengj31@aliyun.com)

    DOI:10.16522/j.cnki.cjph.2025.07.006

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