Tissue Intrinsic Fluorescence Spectrum Recovery Algorithm and Its Application in Diabetes Screening

Yang Zhang; Jingshu Ni; Yuanzhi Zhang; Zhaohui Fang; Yikun Wang; Yong Liu

doi:10.3788/CJL201845.0707001

Chinese Journal of Lasers, Volume. 45, Issue 7, 0707001(2018)

Tissue Intrinsic Fluorescence Spectrum Recovery Algorithm and Its Application in Diabetes Screening

Yang Zhang^1,2, Jingshu Ni¹, Yuanzhi Zhang^{、* *}, Zhaohui Fang³, Yikun Wang^1,2, and Yong Liu¹

¹ Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument, Institute of Applied Technology, Hefei, Anhui 230031, China

² Wan Jiang New Industy Technology Development Center, Tongling, Anhui 244000, China

³ The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui 230031, China

show less

Abstract Get PDF(in Chinese)

Figures & Tables(7)

Fig. 1. Schematics for measuring fluorescence and diffuse reflection spectra of tissue. (a) Fluorescence spectrum; (b) diffuse reflection spectrum

Download full size

Fig. 2. Schematic of spectrum detection system

Download full size

Fig. 3. Spectra of phantom sets with 0.96 mg/mL NADH and varying concentrations of Hb and polystyrene. (a)-(b) Fluorescence spectra; (c)-(d) diffuse reflection spectra of fluorescence excitation band; (e)-(f) diffuse reflection spectra of fluorescence emission band

Download full size

Fig. 4. Relationship between the (a) phantom raw fluorescence or (b) fluorescence spectrum after recovery and mass concentration of NADH

Download full size

Fig. 5. ROC curves of correction spectrum (kx=0.9, km=-0.8) and original spectrum (kx=0, km=0) for diabetes screening

Download full size

Table 1. Composition of single-layer phantom
View table
Table 1. Composition of single-layer phantom
Phantomnumber Mass concentration /(mg·mL^-1)
NADH Hb Polystyrene
1 X 0.5 0.02
2 X 1.0 0.02
3 X 1.5 0.02
4 X 0.5 0.04
5 X 1.0 0.04
6 X 1.5 0.04
7 X 0.5 0.06
8 X 1.0 0.06
9 X 1.5 0.06
10 X 0 0

Table 2. Absorption coefficient μa and scattering coefficientμs at typical excitation and emission wavelengths of NADH
View table
Table 2. Absorption coefficient μa and scattering coefficientμs at typical excitation and emission wavelengths of NADH
Excitation wavelengthof 370 nm Emission wavelengthof 480 nm
μ_a /cm^-1 μ_s /cm^-1 μ_a /cm^-1 μ_s /cm^-1
25 108 2.6 92
50 217 5.2 185
75 326 7.8 277

Tools

Get Citation

Copy Citation Text

Yang Zhang, Jingshu Ni, Yuanzhi Zhang, Zhaohui Fang, Yikun Wang, Yong Liu. Tissue Intrinsic Fluorescence Spectrum Recovery Algorithm and Its Application in Diabetes Screening[J]. Chinese Journal of Lasers, 2018, 45(7): 0707001

Download Citation

EndNote(RIS)BibTex Plain Text

Set citation alerts for article

Save article for my favorites