Laser & Optoelectronics Progress, Volume. 60, Issue 22, 2211005(2023)

Design of Portable Quantitative Phase Microscopy Imaging System

Yingran Zhao1,2、*, Keding Yan2、**, Jiayi Li2, and Yan Ma3
Author Affiliations
  • 1School of Intelligent Science and Information Engineering, Xi'an Peihua University, Xi'an 710125, Shaanxi, China
  • 2School of Electronic Information Engineering, Xi'an Technological University, Xi'an 710021, Shaanxi, China
  • 3School of Electrical Engineering and Information, Southwest Petroleum University, Chengdu 610500, Sichuan, China
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    Figures & Tables(14)
    Schematic diagram for solving the transmission equation of light intensity
    Basic schematic diagram of the system
    System composition block diagram
    Mechanical structure assembly drawing and physical drawing display
    Motor drive circuit wiring schematic diagram
    System interactive software GUI interface and basic flow chart. (a) GUI interface and module distribution; (b) software basic usage flow chart
    Display of field correction results
    System hardware and software joint image
    System resolution test. (a) Physical image of USAF-1951 resolution board; (b) resolution plate image under 10 and 20 times objective lens
    System phase recovery accuracy test. (a) The defocusing image and phase image of the phase recovery plate; (b) three-dimensional phase distribution; (c) a cross-section image cropped along the dotted line
    Phase diagrams and height distribution of Vero cell enzymatic process. (a) Two-dimensional phase distribution diagram; (b) three-dimensional height distribution
    Defocusing image, phase image and height distribution of flat glass(left 1: underfocus, left 2: overfocus, left 3: phase, right 1: height). (a) Scratch defect perspective 1; (b) scratch defect perspective 2
    • Table 1. Detailed parameters for CMOS cameras

      View table

      Table 1. Detailed parameters for CMOS cameras

      Camera indicatorPerformance parameter
      Model of cameraDaheng VEN-134-90U3M-D
      Data interfaceUSB 3.0
      Resolution1280×1024×2
      FPS90
      Pixel size4.8 μm×4.8 μm
    • Table 2. Communication protocol formulation

      View table

      Table 2. Communication protocol formulation

      Name of codeThe meaning of the command
      A=0/A=1X-axis motor is off/enabled
      B=0/B=1Y-axis motor is off/enabled
      X+=n/X-=nX axis motor advances/back n pulses
      Y+=n/Y-=nY axis motor advances/back n pulses
      XS=d/YS=dX/Y axis motor moves to n position in absolute coordinates
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    Yingran Zhao, Keding Yan, Jiayi Li, Yan Ma. Design of Portable Quantitative Phase Microscopy Imaging System[J]. Laser & Optoelectronics Progress, 2023, 60(22): 2211005

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    Paper Information

    Category: Imaging Systems

    Received: Feb. 6, 2023

    Accepted: Mar. 22, 2023

    Published Online: Nov. 16, 2023

    The Author Email: Yingran Zhao (2856497933@qq.com), Keding Yan (yankeding168@163.com)

    DOI:10.3788/LOP230577

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