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Acta Optica Sinica, Volume. 44, Issue 18, 1801010(2024)

Dual-Band Tunable Pulse Light Induced Fluorescence Measurement Technology for Photochemical Quantum Yield of Phytoplankton

Ming Dong1,2,3, Gaofang Yin1,2,3、*, Nanjing Zhao1,2,3、**, Renqing Jia2,3, Mingjun Ma2,3, Peng Huang2,3, Xiang Hu2,3, Xiaoling Zhang4, Xiang Wang5, and Tianhong Liang2,3
Author Affiliations
  • 1College of Environmental Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230026, Anhui , China
  • 2Key Laboratory of Environmental Optics and Technology, Chinese Academy of Sciences, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui , China
  • 3Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province , Hefei 230031, Anhui, China
  • 4Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, Anhui , China
  • 5School of Electrical Engineering, Anhui Polytechnic University, Wuhu 241200, Anhui , China
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    Figures & Tables(6)
    Flow chart of adaptive adjustment of saturation excitation wavelength and light intensity

    Fig. 1. Flow chart of adaptive adjustment of saturation excitation wavelength and light intensity

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    Photochemical quantum yield measurement system for living phytoplankton

    Fig. 2. Photochemical quantum yield measurement system for living phytoplankton

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    Measurement results of Microcystis aeruginosa. (a) Fluorescence kinetics curves of three excitation modes; (b) comparison of FV/FM

    Fig. 3. Measurement results of Microcystis aeruginosa. (a) Fluorescence kinetics curves of three excitation modes; (b) comparison of FV/FM

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    Measurement results of Chlorella pyrenoidosa. (a) Fluorescence kinetics curves of three excitation modes; (b)comparison of FV/FM

    Fig. 4. Measurement results of Chlorella pyrenoidosa. (a) Fluorescence kinetics curves of three excitation modes; (b)comparison of FV/FM

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    Fluorescence kinetics curves of mixed algae with different volume ratios of blue algae and green algae. (a) Volume ratio is 2∶18; (b) volume ratio is 4∶16; (c) volume ratio is 6∶14; (d) volume ratio is 8∶12; (e) volume ratio is 10∶10; (f) volume ratio is 12∶8; (g) volume ratio is 14∶6; (h) volume ratio is 16∶4; (i) volume ratio is 18∶2

    Fig. 5. Fluorescence kinetics curves of mixed algae with different volume ratios of blue algae and green algae. (a) Volume ratio is 2∶18; (b) volume ratio is 4∶16; (c) volume ratio is 6∶14; (d) volume ratio is 8∶12; (e) volume ratio is 10∶10; (f) volume ratio is 12∶8; (g) volume ratio is 14∶6; (h) volume ratio is 16∶4; (i) volume ratio is 18∶2

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    Measurement results of mixed algae FV/FM. (a) Comparison of FV/FM; (b) FV/FM correlation between dual-band excitation and FastOcean sensor

    Fig. 6. Measurement results of mixed algae FV/FM. (a) Comparison of FV/FM; (b) FV/FM correlation between dual-band excitation and FastOcean sensor

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    Ming Dong, Gaofang Yin, Nanjing Zhao, Renqing Jia, Mingjun Ma, Peng Huang, Xiang Hu, Xiaoling Zhang, Xiang Wang, Tianhong Liang. Dual-Band Tunable Pulse Light Induced Fluorescence Measurement Technology for Photochemical Quantum Yield of Phytoplankton[J]. Acta Optica Sinica, 2024, 44(18): 1801010

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    Paper Information

    Category: Atmospheric Optics and Oceanic Optics

    Received: Jan. 18, 2024

    Accepted: Feb. 23, 2024

    Published Online: Sep. 11, 2024

    The Author Email: Yin Gaofang (gfyin@aiofm.ac.cn), Zhao Nanjing (njzhao@aiofm.ac.cn)

    DOI:10.3788/AOS240520

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology