Journal of Radiation Research and Radiation Processing, Volume. 41, Issue 1, 010301(2023)

Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice

Yizhe XUE, Zhaowen ZHANG, Ling GUO, Panpan LAI, Jiajin LIN, Tongzhou QIN, Yuntao JING, Xing WANG, Guiqiang ZHOU, and Guirong DING*
Author Affiliations
  • Department of Radiation Protection Medicine, School of Military Preventive Medicine, Air Force Medical University, Xi'an 710032, China
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    Figures & Tables(8)
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on the general health of mice: (a) body weight of mouse; (b) testis weight; (c) testis coefficient; data are expressed as Mean±SD, n=12
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on the morphological structure of seminiferous tubules in testis tissue of mice: (a) HE staining results of testis tissue, n=3; (b) diameter of seminiferous tubules; (c) thickness of seminiferous epithelium
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on epididymal morphology and sperm quality in a mice: (a) HE staining of epididymal tissue, n=3; (b) sperm count; (c) sperm abnormality rate
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on serum sex hormones in adult male C57 mice: (a) testosterone content; (b) FSH content; (c) LH content; (d) GnRH content, *pvs. Sham; **pvs. Sham; data are presented as Mean±SD, n=11~12
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on the secretory function of sertoli cells in mice: (a) GDNF concentration; (b) SCF concentration; data are presented as Mean±SD, n=6
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on the secretion of related proteins from sertoli cells in mice: (a) representative results of Western blot; (b) relative expression of GDNF; (c) relative expression of SCF; data are presented as Mean±SD, n=3
    Effects of continuous exposure to 4.9 GHz RFR for 21 days on blood-testis barrier tight junction-related proteins in mice: (a) representative bargraphs of Western blot; (b) ZO-1 gray value; (c) occludin gray value; data are expressed as Mean±SD, n=3
    • Table 1. Western blotting primary antibody details

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      Table 1. Western blotting primary antibody details

      抗体名称 Antibody种属来源 Species公司 Company产地 Origin稀释比 Dilution ratio

      抗β-肌动蛋白

      Anti-β-actin

      小鼠单克隆抗体

      Mouse monoclonal Ab

      CMCTAG

      密尔沃基,美国

      Milwaukee, USA

      1∶5 000
      抗神经营养因子兔多克隆抗体Abcam剑桥,英国1∶400
      Anti-GDNFRabbit Polyclonal AbCambridge, England
      抗干细胞因子兔多克隆抗体SAB马里兰州,美国1∶300
      Anti-SCFRabbit Polyclonal AbMaryland, USA

      抗ZO-1

      Anti-ZO-1

      兔多克隆抗体

      Rabbit Polyclonal Ab

      Proteintech武汉1∶1 000
      Wuhan

      抗Occludin

      Anti-Occludin

      兔多克隆抗体

      Rabbit Polyclonal Ab

      Proteintech

      武汉

      Wuhan

      1∶2 000
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    Yizhe XUE, Zhaowen ZHANG, Ling GUO, Panpan LAI, Jiajin LIN, Tongzhou QIN, Yuntao JING, Xing WANG, Guiqiang ZHOU, Guirong DING. Effects of 5G mobile phone radiation on sperm quality and sex hormone levels in mice[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(1): 010301

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    Paper Information

    Category: Research Articles

    Received: Jun. 24, 2022

    Accepted: Oct. 17, 2022

    Published Online: Mar. 6, 2023

    The Author Email:

    DOI:10.11889/j.1000-3436.2022-0062

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