Laser & Optoelectronics Progress, Volume. 62, Issue 18, 1817014(2025)
Progress and Challenges of NIR-II Fluorescent Probes in the Detection of Mitochondria-Associated Markers (Invited)
Mitochondria are the core organelles of cell energy metabolism and homeostasis regulation, and are closely related to neurodegenerative diseases, cancer and metabolic diseases. Accurate detection of mitochondrial markers is essential for early diagnosis, mechanism research and curative effect evaluation of disease. Near-infrared second window (NIR-II, 1000?1700 nm) fluorescence imaging technology has become a leading tool for real-time dynamic monitoring of mitochondrial markers with the advantages of deep tissue penetration, low autofluorescence interference and high temporal and spatial resolution. This paper systematically reviews the research progress of NIR-II fluorescent probe in the detection of mitochondrial markers, including the detection strategies of reactive oxygen species (ROS), membrane potential (MMP), microenvironment pH and metabolic molecules [ATP, NAD(P)h, etc.]. The related probes targeted mitochondria specific structures (such as triphenylphosphine cation) to locate mitochondria, and combined with ratio design, FRET/BRET energy transfer and other strategies to improve the detection sensitivity and signal-to-noise ratio. For example, Mito-Cy-Tfs (ROS detection), MOF based ATP response probe and pH sensitive Fe-CDs have all achieved accurate visualization of markers in living cells and
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Qinghui Wang, Chenglong Zhang, Kai Li, Rui Xin, Liangcan He, Shaoqin Liu. Progress and Challenges of NIR-II Fluorescent Probes in the Detection of Mitochondria-Associated Markers (Invited)[J]. Laser & Optoelectronics Progress, 2025, 62(18): 1817014
Category: Medical Optics and Biotechnology
Received: May. 16, 2025
Accepted: Aug. 6, 2025
Published Online: Sep. 12, 2025
The Author Email: Rui Xin (xinrui0906@hit.edu.cn), Liangcan He (liangcanhe@hit.edu.cn)
CSTR:32186.14.LOP251239