Acta Photonica Sinica, Volume. 54, Issue 3, 0318002(2025)

Development of 30× Fluorescence Detection Microscope for Particle Image Velocimetry Measurement

Taiyuange LOU, Chengxiang GUO, Tong YANG, Lei YANG*, and Hongbo XIE
Author Affiliations
  • Key Laboratory of Optoelectronics Information Technology(Ministry of Education),School of Precision Instruments and Optoelectronics Engineering,Tianjin University,Tianjin 300072,China
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    Figures & Tables(10)
    Principle of long working distance microscope objective design(unit:mm)
    Schematic of the micro-PIV system
    Initial structure of the microscopic objective[11]
    Design of microscopic objective
    The experimental setups of the micro-PIV systems
    Image of a USAF standard resolution target
    Particle shooting experiment
    Result of our method
    • Table 1. Microscope design specifications

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      Table 1. Microscope design specifications

      ParametersSpecifications
      Work spectrum400~800 nm
      Magnification30
      Numerical aperture0.35
      Working distance>15 mm
      Depth of field>1.5 μm
      Distortion<0.3%
    • Table 2. Distribution of diffraction MTF modulus values at 500 lp/mm for 1 500 simulated systems

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      Table 2. Distribution of diffraction MTF modulus values at 500 lp/mm for 1 500 simulated systems

      PercentValue
      90%0.235 030 11
      80%0.290 422 90
      50%0.321 431 40
      20%0.412 800 03
      10%0.440 162 81
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    Taiyuange LOU, Chengxiang GUO, Tong YANG, Lei YANG, Hongbo XIE. Development of 30× Fluorescence Detection Microscope for Particle Image Velocimetry Measurement[J]. Acta Photonica Sinica, 2025, 54(3): 0318002

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    Paper Information

    Category: Microscopy

    Received: Sep. 19, 2024

    Accepted: Nov. 22, 2024

    Published Online: Apr. 22, 2025

    The Author Email: Lei YANG (yanglei@tju.edu.cn)

    DOI:10.3788/gzxb20255403.0318002

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