Strains are the foundation for the development of the edible and medicinal fungi industry, and its quality directly affects the industrial benefits. Aiming at the problems of chaotic Ganoderma strains and strain degradation in Northern Guangdong Region, the experiment carried out qualitative and quantitative comparisons and comprehensive analyses on the biological characteristics of 8 Ganoderma strains collected from Northern Guangdong Region. These characteristics included the strains' antagonistic reactions, heat resistance, mold resistance, growth status and rate of mycelia, growth period, and biological efficiency, through comparison and analysis, high-quality strains suitable for Northern Guangdong Region were screened out. The results showed that the 8 strains belonged to at least 5 Ganoderma species. GL1 and GL8 were excellent strains more suitable for cultivation in Northern Guangdong Region due to their good mycelial growth, fast growth rate, strong stress resistance, short growth period, and high biological efficiency. GL5 had strong stress resistance and high temperature tolerance, showing certain application value and being suitable for summer-autumn cultivation in Northern Guangdong Region.