Chinese Journal of Lasers, Volume. 50, Issue 3, 0307104(2023)

Advances in Rapid Three-Dimensional Wide Field Microscopy

Jingrong Ren1,2、†, Xiangda Fu1,2、†, Mengrui Wang1,2, Tianyu Zhao1,2, Zhaojun Wang1,2, Kun Feng1,2, Yansheng Liang1,2, Shaowei Wang1,2, and Ming Lei1,2、*
Author Affiliations
  • 1MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, Xi an 710049, Shaanxi, China
  • 2School of Physics, Xi an Jiaotong University, Xi an 710049, Shaanxi, China
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    Figures & Tables(11)
    Imaging principle of SFF. (a) Schematic of SFF system [25]; (b) lens imaging model when object is moving[26]
    Imaging results of SFF. (a) Image sequence of sunken plane with NSST[42]; (b) image sequence of coins[42]; (c)-(e) comparison of SFF results on TFT-LCD color filter[48]
    Schematics of SIM system based on DMD projection and LED lighting[65]. (a) Schematic of TIR prism; (b) structure of system, in which ① is LED light guide, ② is DMD, ③ is sample stage, and ④ is CCD camera
    Optical sectioning SIM imaging results and imaging devices. (a) 3D imaging results of lunar meteorite[74]; (b) 3D imaging results of amber[73]; (c) schematic of imaging system[73]; (d) physical picture of imaging device[73]
    Imaging results of azurite[76]. (a)-(c) Three-view maximum projection images of azurite; (d) 3D height map of Fig. 5 (a) and height fluctuation distribution on black line segment; (e) detail image on malachite; (f) image of malachite restored by HSV decoding algorithm; (g) detail image on white crystal; (h) image of white crystal restored by HSV decoding algorithm
    GANs for image reconstruction in microscopy[88]
    3D imaging of caenorhabditis elegans head neuron nuclei obtained using Deep-Z[15]
    Volumetric imaging based on Recurrent-MZ[16]. (a) Recurrent-MZ volumetric imaging framework; (b) Recurrent-MZ network structure
    Overview of WFCON-Net architecture[89]. (a) WFCON-Net based on 3D GAN; (b) network architecture of generator;
    Image processing results of deep learning model. (a)-(d) Typical coronal maximum intensity projections of four mouse brains[90]; (e) application of cGAN model in radical prostatectomy[91]
    Schematics of FC-WFM-Deep imaging system[92]. (a) Training network using wide field (WF) image and corresponding composite optical sectioning (OS) reference image; (b) reconstructing high-quality output image consisting of OS in DOF using trained network
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    Jingrong Ren, Xiangda Fu, Mengrui Wang, Tianyu Zhao, Zhaojun Wang, Kun Feng, Yansheng Liang, Shaowei Wang, Ming Lei. Advances in Rapid Three-Dimensional Wide Field Microscopy[J]. Chinese Journal of Lasers, 2023, 50(3): 0307104

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    Paper Information

    Category: Biomedical Optical Imaging

    Received: Oct. 8, 2022

    Accepted: Nov. 2, 2022

    Published Online: Feb. 6, 2023

    The Author Email: Lei Ming (ming.lei@mail.xjtu.edn.cn)

    DOI:10.3788/CJL221303

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