Fig. 1. OCT system and zebrafish samples. (a) Schematic of OCT setup and photograph of zebrafish hold by agar; (b) photograph of zebrafish samples from three age groups

Fig. 2. OCT in vivo imaging of zebrafish head. (a) Continuous B-scan OCT images; (b) constructed three-dimensional OCT images; (c) virtual segmentation results of three-dimensional OCT images

Fig. 3. Coronal views of images of zebrafish brains from three age groups. (a) OCT images; (b) sectional staining images

Fig. 4. Horizontal views of images of zebrafish brains from three age groups. (a) OCT images; (b) sectional staining images

Fig. 5. Measured brain volume of zebrafish. (a) Three dimensional images of zebrafish brains; (b) measured results of brain volume

Fig. 6. Brain area of zebrafish obtained based on Fig. 4. (a)--(c) Comparison of sectional staining results and OCT results; (d)--(f) enlarged sectional staining results