Self-Adaptive Mixed-Emitter Single-Molecule Localization Algorithm

Fig. 1. Reconstructed images of two 200 nm stripes apart generated by different algorithms, density of the strip is 1 $μ m^{- 2}$ (above) and 51 $μ m^{- 2}$ (below), respectively

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Fig. 2. Flowchart of SM algorithm, where the process of binary SNR generation, emitter identification and self-adaptation fitting algorithm are represented by yellow, blue and orange arrows, respectively

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Fig. 3. Simulation data and algorithm comparison on simulation data. (a) Simulated images with different densities; (b) comparison of metrics of different algorithms; (c) localization error of different algorithms; (d) simulated reference image (ground truth,GT); (e) sectional intensity profiles and local magnification of reconstructed super-resolution images by different algorithms

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Fig. 4. Algorithm comparison on experiment data. (a) rFRC maps of super-resolution images obtained from different algorithms; (b)‒(c) local magnification of super resolution images; (d) sectional intensity profile corresponding to white lines in Fig. 4(b)

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Table 1. Comparison of super-resolution images recovered from different algorithms with ground truth image
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Table 1. Comparison of super-resolution images recovered from different algorithms with ground truth image
Algorithm RMSE SSIM PSNR
SE 1.18×10³ 0.559 21
SM 0.78×10³ 0.699 23.93
ME 1.16×10³ 0.34 21.15

Table 2. Quantitative resolution features of different algorithms given by rFRC
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Table 2. Quantitative resolution features of different algorithms given by rFRC
Algorithm Min /nm Mean /nm Max /nm
SE 19.4 42.22 551.39
SM 19.4 34.45 100.82
ME 19.85 44.14 200.23

Table 3. Comparison of SE, ME and SM algorithms
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Table 3. Comparison of SE, ME and SM algorithms
Algorithm Mismatch error Crosstalk error Advantage density Photo utilization efficiency Computational complexity Speed
SE None Much Low Low Simple High
ME Much Little High High Complex Low
SM None None Full High Simple Middle

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Yizhe Liu, Weisong Zhao, Yuzhen Liu, Haoyu Li. Self-Adaptive Mixed-Emitter Single-Molecule Localization Algorithm[J]. Chinese Journal of Lasers, 2023, 50(21): 2107106

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