Fig. 1. Principle of double-ring beam reshaping and its comparative performance. (a) Lowsidelobe Bessel light sheet obtained by the modulation of a double-ring mask; (b) characterization and comparison of performance of our multi-scale low sidelobe light sheet

Fig. 2. Schematic diagrams of the proposed system and the timing control flow charts. (a) The optical design of our multi-scale light sheet fluorescence microscope; (b) the control charts for different imaging applications

Fig. 3. Comparison of imaging results between single-ring Bessel light sheet and DR-SPIM. (a) x-z plane max intensity projection of point spread function by 2.5 μm single-ring Bessel light sheet with 73% side lobes; (b) x-z plane max intensity projection of point spread function by 2.5 μm DR-SPIM with 18% side lobes

Fig. 4. 3D dynamics of mitochondrial outer membrane in U2OS cell. (a) Three-dimensional distribution of mitochondrial outer membrane in a whole live cell, scale bar: 10 μm; (b) the movement of local mitochondrial outer membrane, scale bar: 2 μm

Fig. 5. Interactions between mitochondrial outer membrane and Drp1 in U2OS cell. (a) Three-dimensional co-localization of mitochondrial outer membrane and Drp1, scale bar: 3 μm; (b) interactions between mitochondrial outer membrane and Drp1 in a region of interest, scale bar: 1 μm

Fig. 6. 3D visualization of multi-scale imaging results. (a) 3D visualization and (b) axial 3D view of an expanded nuclear hole (expansion factor: 4), scale bar: 10 μm; (c) 3D visualization of a selected small region, scale bar: 2 μm; max intensity projection of the (d) x-y plane and (e) y-z plane of the region, scale bar: 1 μm; (f) 3D visualization of a whole mouse brain, scale bar: 1 mm; 3D visualization of a region of interest in (g) cortex and (h) thalamus, scale bar: 100 μm