[1] [1] Manz A，Graber N，Widmer H M，et al. Miniaturized total chemical analysis systems：A novel concept for chemical sensing [J]. Sensors and Actuators B-chemical(S0925-4005)，1990，1(1)：244-248.

[2] [2] FANG Zhaolun，FANG Qun. Developments and Trends of Microfluidic Chip Analytical Systems [J]. Modernentific Instruments，2001，4(3/6)：1-2.

[3] [3] DAI Xudong. The Study of the pressure effect on separation repeatability in microfluidic chip [D]. Dalian：Dalian University of Technology，2009：5-6.

[4] [4] LIU Fan，YU Shuxin，TANG Tao，et al. Development and evaluation of a novel three-dimensional adjustment confocal laser-induced fluorescence detector [J]. Chinese Journal of Chromatography，2011，29(9)：896-900.

[5] [5] LIU Yunliang. Laser induced fluorescence detector based on the confocal principle [D]. Dalian：Dalian University of Technology，2012：19-23.

[6] [6] LI Wei. The study of confocal laser induced fluorescence detection device [D]. Dalian：Dalian University of Technology，2006： 9-16.

[7] [7] ZHANG Ying，ZHANG Xin，SHI Guangwei，et al. Application of liquid lens in zoom systems [J]. Chinese Optics，2013， 6(1)：46-56.

[8] [8] GAO Zan. The study of accuracy of the automatic focusing evaluation function and stability [D]. Jinan：Shandong University， 2007：32-34.

[9] [9] CAO Maoyong，SUN Nongliang，YU Daoyin. Digital image evaluation function based on gray gradient [J]. Opto-Electronic Engineering，2003，30(4)：69-72.

[10] [10] ZHAO Hui，BAO Getang，TAO Wei. Experimental research and analysis of automatic focusing function for imaging measurement [J]. Optics and Precision Engineering，2004，12(5)：531-536.

[11] [11] ZHANG Yanchao，ZHAO Jian，HAN Xizhen，et al. Application of SUSAN definition evaluation function in auto-focusing [J]. Chinese Optics，2014，7(2)：240-244.

[12] [12] LI Huiguang，WANG Shuai，SHA Xiaopeng，et al. Study of Auto Focusing Technique of Micro-vision System [J]. Opto-Electronic Engineering，2014，41(8)：1-9.

[13] [13] Blum M G，Bueler M，Gratzel C，et al. Compact optical design solutions using focus tunable lenses [J]. Proceedings of SPIE(S0277-786X)，2011，8167(1)：2.

[14] [14] REN Sigang，LI Jianwei，XIE Lili. Automatic focusing technique based on gray scale difference method [J]. Opto-Electronic Engineering，2003，30(2)：53-55.