Effect of high-energy electron beam irradiation sterilization on the quality of Glycyrrhizae Radix et Rhizoma

Guisen KANG; Liangcai WANG; Yunxia HU; Fangli HU; Haijun WANG; Bingkun LIU; Yuanmeng WANG; Zhijun XIN; Xiao LIU; Xihong LU; Xuehu LI; Libin ZHOU

doi:10.11889/j.1000-3436.2024-0108

Journal of Radiation Research and Radiation Processing, Volume. 43, Issue 3, 030405-1(2025)

Effect of high-energy electron beam irradiation sterilization on the quality of Glycyrrhizae Radix et Rhizoma

Guisen KANG^1,2, Liangcai WANG^1,3, Yunxia HU^1,4, Fangli HU^1,3, Haijun WANG⁵, Bingkun LIU⁵, Yuanmeng WANG^1,3, Zhijun XIN^1,2, Xiao LIU^1,2, Xihong LU^1,2, Xuehu LI^1,2、*, and Libin ZHOU^1,2,6、**

Author Affiliations

¹Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China

²University of Chinese Academy of Sciences, Beijing 100049, China

³Gansu University of Chinese Medicine, Lanzhou 730000, China

⁴Lanzhou University of Technology, Lanzhou 730050, China

⁵Gansu Puankang Pharmaceutical Co.,Ltd., Lanzhou 730207, China

⁶Key Laboratory of Heavy Ion Science and Technology, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China

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Figures & Tables(9)

Fig. 1. HPLC profiles of reference substance of Glycyrrhizae Radix et Rhizoma

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Fig. 2. HPLC fingerprint of Glycyrrhizae Radix et Rhizoma

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Fig. 3. Effect of the antioxidant capacity of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation: (a) ABTS free radical scavenging rate; (b) superoxide anion free radical scavenging rate; (c) hydroxyl scavenging activity; (d) T-AOC (different lowercase letters indicate significant differences among treatment groups (p < 0.05))

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Table 1. HPLC linear regression equation of six compounds in Glycyrrhizae Radix et Rhizoma

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Table 1. HPLC linear regression equation of six compounds in Glycyrrhizae Radix et Rhizoma

化合物 Compounds	回归方程 Linear regression equations	线性范围 / (μg·mL^-1) Linearity range	R²
甘草苷 Liquiritin	Y = 34 406X - 4 471.3	1.54 ~ 48.33	0.999 4
异甘草苷 Isoliquiritoside	Y = 10 767X + 5 858.6	1.25 ~ 40.00	0.999 7
甘草素 Glycyrrhizin	Y = 58 478X - 529.63	0.67 ~ 21.67	0.999 9
甘草酸 Glycyrrhizinic acid	Y = 1 445X - 392.33	3.28 ~ 105.00	0.999 9
异甘草素 Isoglycyrrhizin	Y = 9 870.4X + 872.94	0.36 ~ 11.67	0.999 5
甘草查尔酮A Licochalcone A	Y = 19 365X - 12 973	1.30 ~ 41.67	0.999 5

Table 2. Effect of the microbial loads of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation

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Table 2. Effect of the microbial loads of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation

吸收剂量 / kGy Absorbed dose	微生物指标 / (CFU·g^-1) Microbiological indicator
吸收剂量 / kGy Absorbed dose	需氧菌总数 Total aerobic microbial count	霉菌及酵母菌总数 Total yeast and mold count	耐热菌总数 Total heat- resistant bacteria count
0	5.95×10⁴	2.15×10⁴	8.50×10³
2	3.50×10³	5.00×10²	< 10
4	< 10	< 10	< 10
6	< 10	< 10	< 10
8	< 10	< 10	< 10
10	< 10	< 10	< 10

Table 3. Effect of the color of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation

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Table 3. Effect of the color of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation

吸收剂量 / kGy Absorbed dose	色泽Color
吸收剂量 / kGy Absorbed dose	L值 L value	a值 avalue	b值 bvalue	C值 Cvalue	ΔE值 ΔEvalue
0	82.16 ± 0.07^a	-1.11 ± 0.06^c	18.42 ± 0.24^c	18.45 ± 0.24^c	-
2	80.72 ± 0.04^b	-0.97 ± 0.06^b	19.31 ± 0.12^b	19.34 ± 0.12^b	1.70 ± 0.07
4	79.77 ± 0.04^c	-0.97 ± 0.01^b	19.69 ± 0.13^a	19.71 ± 0.13^a	2.70 ± 0.07
6	79.72 ± 0.05^cd	-0.98 ± 0.04^b	19.63 ± 0.03^ab	19.65 ± 0.03^ab	2.99 ± 0.04
8	79.71 ± 0.05^d	-0.90 ± 0.05^a	19.75 ± 0.16^a	19.77 ± 0.16^a	2.88 ± 0.08
10	79.68 ± 0.05^d	-0.85 ± 0.04^a	19.72 ± 0.17^a	19.74 ± 0.16^a	2.84 ± 0.30
CV	0.06	4.43	1.08	1.08	-

Table 4. Effect of the physicochemical property of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation

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Table 4. Effect of the physicochemical property of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation

吸收剂量 / kGy Absorbed dose	理化性质 Physical and chemical property
吸收剂量 / kGy Absorbed dose	水分 / % Moisture	醇溶性浸出物含量 / % Ethanol-soluble extracts content
0	9.42 ± 0.10^a	36.17 ± 0.54^a
2	9.57 ± 0.08^a	36.49 ± 1.26^a
4	9.56 ± 0.10^a	36.56 ± 0.57^a
6	9.55 ± 0.14^a	36.59 ± 0.38^a
8	9.43 ± 0.09^a	36.63 ± 0.98^a
10	9.44 ± 0.11^a	36.76 ± 0.39^a

Table 5. Effect of the content of active ingredients in Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation

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Table 5. Effect of the content of active ingredients in Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation

吸收剂量 / kGy Absorbed dose	活性成分 / % Active ingredients content
吸收剂量 / kGy Absorbed dose	甘草苷 Liquiritin	异甘草苷 Isoliquiritoside	甘草素 Glycyrrhizin	甘草酸 Glycyrrhizinic acid	异甘草素 Isoglycyrrhizin	甘草查尔酮A Licochalcone A	总黄酮含量 Total content of flavonoids
0	1.06 ± 0.05^b	0.69 ± 0.05^a	0.08 ± 0.06^a	4.18 ± 0.06^b	0.09 ± 0.02^a	0.38 ± 0.02^a	1.91 ± 0.03^a
2	1.06 ± 0.06^b	0.70 ± 0.04^a	0.08 ± 0.04^a	4.18 ± 0.01^b	0.09 ± 0.01^a	0.38 ± 0.02^a	1.92 ± 0.01^a
4	1.06 ± 0.01^b	0.70 ± 0.08^a	0.09 ± 0.02^a	4.18 ± 0.03^b	0.09 ± 0.03^a	0.38 ± 0.01^a	1.92 ± 0.01^a
6	1.06 ± 0.03^b	0.69 ± 0.07^a	0.09 ± 0.01^a	4.18 ± 0.02^b	0.09 ± 0.01^a	0.38 ± 0.01^a	1.92 ± 0.02^a
8	1.09 ± 0.02^a	0.69 ± 0.02^a	0.08 ± 0.01^a	4.38 ± 0.05^a	0.09 ± 0.02^a	0.38 ± 0.01^a	1.92 ± 0.04^a
10	1.09 ± 0.02^a	0.69 ± 0.04^a	0.08 ± 0.02^a	4.38 ± 0.03^a	0.09 ± 0.03^a	0.38 ± 0.01^a	1.90 ± 0.01^a

Table 6. HPLC similarity of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation with different doses

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Table 6. HPLC similarity of Glycyrrhizae Radix et Rhizoma treated by electron beam irradiation with different doses

样品 Sample	0 kGy	2 kGy	4 kGy	6 kGy	8 kGy	10 kGy	对照谱图R Reference spectrum R
0 kGy	1.000	0.987	0.988	0.998	0.989	0.996	0.997
2 kGy	0.987	1.000	0.989	0.986	0.987	0.987	0.993
4 kGy	0.988	0.989	1.000	0.986	0.998	0.991	0.996
6 kGy	0.998	0.986	0.986	1.000	0.987	0.994	0.996
8 kGy	0.989	0.987	0.998	0.987	1.000	0.988	0.996
10 kGy	0.996	0.987	0.991	0.994	0.988	1.000	0.997
对照谱图R Reference spectrum R	0.997	0.993	0.996	0.996	0.996	0.997	1.000

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Guisen KANG, Liangcai WANG, Yunxia HU, Fangli HU, Haijun WANG, Bingkun LIU, Yuanmeng WANG, Zhijun XIN, Xiao LIU, Xihong LU, Xuehu LI, Libin ZHOU. Effect of high-energy electron beam irradiation sterilization on the quality of Glycyrrhizae Radix et Rhizoma[J]. Journal of Radiation Research and Radiation Processing, 2025, 43(3): 030405-1

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Paper Information

Category: RADIATION TECHNOLOGY APPLICATION

Received: Dec. 2, 2024

Accepted: Feb. 10, 2025

Published Online: Jul. 7, 2025

The Author Email: Xuehu LI (李雪虎), Libin ZHOU (周利斌)

DOI:10.11889/j.1000-3436.2024-0108

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology