Transcriptome Characteristics Analysis of Terahertz Wave Inhibited Leukemia K562 Cells Based on RNA Sequencing

Zhong Chen; Guangbin Shang; Liu Fu; Ge Liu; Xuan Chen; Qi Zhang; Xiaonan Lu

doi:10.3788/LOP202259.0717001

Laser & Optoelectronics Progress, Volume. 59, Issue 7, 0717001(2022)

Transcriptome Characteristics Analysis of Terahertz Wave Inhibited Leukemia K562 Cells Based on RNA Sequencing

Zhong Chen^1,2, Guangbin Shang^1,2, Liu Fu^1,2, Ge Liu^1,2, Xuan Chen^1,2, Qi Zhang³, and Xiaonan Lu^3、*

¹Research Center for Differention and Development of TCM Basic Theory, Jiangxi University of Chinese Medicine, Nanchang , Jiangxi 330004, China

²Jiangxi Province Key Laboratory of TCM Etiopathogenisis, Nanchang , Jiangxi 330004, China

³Experimental Center of Basic Medicine, Jiangxi University of Chinese Medicine, Nanchang , Jiangxi 330004, China

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Figures & Tables(10)

Fig. 1. Effect of terahertz wave on K562 cell viability

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Fig. 2. Correlation analysis of samples. (a) Correlation between expressed genes of the blank control group and the intervention group; (b) PCA result of expressed genes of the blank control group and the intervention group

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Fig. 3. Changes of genes in K562 cells after terahertz wave intervention. (a) Volcano plot of differentially expressed genes; (b) heatmap of top 50 differentially expressed genes by |log₂(X_FC)|

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Fig. 4. Terahertz wave interferes with GO function of K562 cells differentially expressed genes. (a) Up-regulated differentially expressed genes; (b) down-regulated differentially expressed genes

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Fig. 5. GO enrichment analysis of K562 up-regulated differentially expressed genes by terahertz wave intervention

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Fig. 6. GO enrichment analysis of K562 down-regulated differentially expressed genes by terahertz wave intervention

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Fig. 7. KEGG pathway enrichment analysis of differentially expressed genes in K562 cells by terahertz wave intervention. (a) Up-regulated differentially expressed genes; (b) down-regulated differentially expressed genes

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Fig. 8. KEGG pathway enrichment chord diagram for differentially expressed genes. (a) Up-regulated differentially expressed genes; (b) down-regulated differentially expressed genes

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Fig. 9. Core network of protein-protein interactions of differentially expressed genes in K562 cells by terahertz wave intervention

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Table 1. Quality assessment of the sequencing data

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Table 1. Quality assessment of the sequencing data

Sample	Clean reads	Clean bases /GB	Error rate /%	Q20 /%	Q30 /%	GC /%
K1	48409502	7.10	0.0246	98.12	94.58	52.99
K2	63665332	9.37	0.0245	98.20	94.68	53.54
K3	60913510	8.94	0.0243	98.27	94.89	53.23
T1	63921046	9.27	0.0243	98.29	94.93	54.00
T2	53271322	7.79	0.0242	98.29	94.94	53.72
T3	56105090	8.21	0.0246	98.12	94.61	54.03

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Zhong Chen, Guangbin Shang, Liu Fu, Ge Liu, Xuan Chen, Qi Zhang, Xiaonan Lu. Transcriptome Characteristics Analysis of Terahertz Wave Inhibited Leukemia K562 Cells Based on RNA Sequencing[J]. Laser & Optoelectronics Progress, 2022, 59(7): 0717001

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Paper Information

Category: Medical Optics and Biotechnology

Received: Jun. 1, 2021

Accepted: Jun. 28, 2021

Published Online: Apr. 7, 2022

The Author Email: Xiaonan Lu (luxn2011@126.com)

DOI:10.3788/LOP202259.0717001

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology