Infrared and Laser Engineering, Volume. 54, Issue 8, 20250136(2025)

Method and validation in second near-infrared window for multi-scale fluorescence intravital imaging

Chenglei YE1,2, Zhiping HE1, Xuehan WANG1,2, and Aijun JIN1、*
Author Affiliations
  • 1Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
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    Figures & Tables(4)
    The method of multi-scale NIR-II fluorescence intravital imaging
    Multi-scale NIR-II fluorescence intravital imaging device. (a) Diagram of the multi-scale NIR-II fluorescence imaging intravital device, which is divided into macro imaging modules and micro imaging modules; (b) Diagram of the multi-scale NIR-II fluorescence intravital imaging system model; (c)-(d) Working diagrams of macroscopic and microscopic imaging, respectively
    The experiment results of Multi-Scale NIR-II fluorescence intravital imaging in vivo. (a) Images of macroscopic observations of control laboratory rat with non-fluorescent livers; (b) Images of macroscopic observations of laboratory rat with fluorescently labeled livers; (c) Microscopic imaging of fluorescently labeled liver tissue in living laboratory rat; (d) The images obtained from the 12-row ×15-column traversal scan of the liver of living laboratory rat were spliced; (e) The microscopic global image of the liver; (f) Relationship between microscopic images of liver and laboratory rat
    Block matching image stitching method based on gray-level information
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    Chenglei YE, Zhiping HE, Xuehan WANG, Aijun JIN. Method and validation in second near-infrared window for multi-scale fluorescence intravital imaging[J]. Infrared and Laser Engineering, 2025, 54(8): 20250136

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    Paper Information

    Category: Optical imaging, display and information processing

    Received: Mar. 4, 2025

    Accepted: --

    Published Online: Aug. 29, 2025

    The Author Email: Aijun JIN (jin_aijun@163.com)

    DOI:10.3788/IRLA20250136

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