Chinese Journal of Lasers, Volume. 45, Issue 4, 407003(2018)

Rapid Microscopic Spectral Imaging of Lung Cancer Tissue Based on Acousto-Optic Tunable Filter

Yuan Jiangwei, Zhang Chunguang*, Wang Hao, and Shi Lei
Author Affiliations
  • [in Chinese]
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    Figures & Tables(9)
    Vectorial diagram of noncollinear acoustic-optic interaction
    Experimental setup of the AOTF-based microscopic spectral imaging system
    Diffraction spectra of AOTF at the different acoustic frequencies. (a) 127 MHz; (b) 134 MHz; (c) 148 MHz; (d) 155 MHz; (e) 162 MHz; (f) 169 MHz
    Performance of AOTF-based microscopic spectral imaging system. (a) Relationship between spectral bandwidth of diffracted beam and central optical wavelength; (b) frequency tuning relation of noncollinear AOTF
    Hyperspectral images of lung cancer tissue with different center wavelengths of diffraction beams. (a) Without filtering; (b) 629.20 nm; (c) 576.21 nm; (d) 567.91 nm; (e) 542.12 nm; (f) 529.45 nm; (g) 503.84 nm; (h) 482.85 nm
    Relationship between the hyperspectral image intensity of the lung cancer tissue and the diffraction light center wavelength
    Intensity difference curves of target area and adjacent tissue
    • Table 1. Main design parameters of AOTF

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      Table 1. Main design parameters of AOTF

      ItemDesign parameterand performance
      Acoustic polar angle θa80.0°
      Incident polar angle θi23.80°
      Range of the optical wavelength400-700 nm
      Range of acoustic frequency fa107.5-233.5 MHz
      Driving power1.0-1.5 W
      Designed diffraction efficiency>75% at 632.8 nm
      Incident aperture angle2.85°
      Length of acousto-optic interaction L4.0 mm
      Spectral bandwidth2.9 nm at 632.8 nm
    • Table 2. Parameters of diffraction light spectra under different ultrasonic frequencies

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      Table 2. Parameters of diffraction light spectra under different ultrasonic frequencies

      Acoustic frequency /MHz127134148155162169
      Central optical wavelength /nm601.28576.21534.08516.10499.92485.19
      Spectral bandwidth /nm1.491.220.980.930.830.79
      Spectral resolution R403.54472.30544.98554.94602.31614.16
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    Yuan Jiangwei, Zhang Chunguang, Wang Hao, Shi Lei. Rapid Microscopic Spectral Imaging of Lung Cancer Tissue Based on Acousto-Optic Tunable Filter[J]. Chinese Journal of Lasers, 2018, 45(4): 407003

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Jul. 18, 2017

    Accepted: --

    Published Online: Apr. 13, 2018

    The Author Email: Chunguang Zhang (cgzhang@fjnu.edu.cn)

    DOI:10.3788/CJL201845.0407003

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