Compared with the traditional beam-splitting elements, the noncollinear acousto-optic tunable filter (AOTF) has many special merits, such as small size, high stability, flexible and easy to tune, convenient for signal reception and processing. It has high application value in spectral imaging field. In this study, the noncollinear AOTF is connected with the converted microscope, and a hyperspectral microscopy imaging system is built. With the system, the rapid microscopic spectral imaging for lung cancer tissue is studied in the visible range. In the experiments, the relationship between the acoustic frequency and the diffracted optical wavelength loaded on AOTF is got, and the theoretical results coincide well with the experimental data. A series of microscopy images and corresponding narrow band spectra of lung cancer tissue are received at central wavelength. The results indicate that the system keeps a well spectral resolution performance in the working waveband. By comparing the lung cancer tissue images under different wavelengths, it is found that obvious image drift is not observed, which indicates that the image has high stability. Lung cancer tissue images of each central wavelength all present good clarity. The comparison of lung cancer tissue image and the analysis results of luminance curve and transmissivity curve show that the best contrast and clarity performance of the images are in the range of 503.45-590.12 nm. It is mainly because the different intrinsic constituents and structures in different areas induce different absorptivities of the signals with different optical wavelengths in lung cancer tissues.