At present, most high-efficiency organic-inorganic halide perovskite (OIHP) solar cells are made of polycrystalline perovskite films, and the surface or grain boundaries of these polycrystalline films often contain a large number of defects. The defects in the OIHP film will lead to non-radiative recombination of photogenerated carriers and induce the decomposition of the OIHP material, resulting in a decrease in the power conversion efficiency (PCE) and stability of the device. In this review, the types of defects in perovskite solar cells and the effects of defects on the performance of perovskite solar cells (PSCs) were analyzed. The progress of passivation of interface defects between electrode and charge transport layer or between charge transport layer and perovskite layer to obtain higher efficiency and stability of perovskite solar cells is introduced in detail. The design ideas of passivation molecules and the challenges facing the commercial application of perovskite photovoltaics are prospected.