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Chinese Journal of Lasers, Volume. 49, Issue 24, 2407202(2022)

Registration Method of Microscopic Bright Field and Fluorescence Synchronous Measurement Images of Phytoplankton Cells

Renqing Jia1,2, Gaofang Yin1,2、*, Nanjing Zhao1,2、**, Min Xu2, Xiang Hu3, Peng Huang3, Tianhong Liang2, Qianfeng He4, Xiaowei Chen2, Tingting Gan2, Xiaoling Zhang5, and Mingjun Ma2
Author Affiliations
  • 1School of Environment Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230026, Anhui, China
  • 2Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optic and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China
  • 3Hefei University, Hefei 230601, Auhui, China
  • 4Anhui Hefei Ecological Environment Monitoring Center, Hefei 230088, Anhui, China
  • 5Anhui University, Hefei 230601, Anhui, China
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    AbstractGet PDF(in Chinese)
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    References (19)
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    Figures & Tables(8)
    Dual optical path imaging system

    Fig. 1. Dual optical path imaging system

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    Flow chart of bright field and fluorescence images registration method

    Fig. 2. Flow chart of bright field and fluorescence images registration method

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    Translation and normalized mutual information. (a) Gray image of bright field and fluorescence; (b) bright field S channel and fluorescence gray image; (c) binarized gray image of bright field and fluorescence; (d) binarization of bright field S channel and fluorescence gray image

    Fig. 3. Translation and normalized mutual information. (a) Gray image of bright field and fluorescence; (b) bright field S channel and fluorescence gray image; (c) binarized gray image of bright field and fluorescence; (d) binarization of bright field S channel and fluorescence gray image

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    Rotation angle and normalized mutual information

    Fig. 4. Rotation angle and normalized mutual information

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    Normalized mutual information after wavelet decomposition. (a) Low frequency component; (b) horizontal high frequency component; (c) vertical high frequency component; (d) diagonal high frequency component

    Fig. 5. Normalized mutual information after wavelet decomposition. (a) Low frequency component; (b) horizontal high frequency component; (c) vertical high frequency component; (d) diagonal high frequency component

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    Bright field and fluorescence images of cells of Scenedesmus sp., Selenastrum capricornutum and Nostoc sp. (a) Bright images; (b) fluorescence images; (c) binarization images of low frequency component of three-level wavelet decomposition in bright field S channel; (d) binarization images of low frequency component of three-level wavelet decomposition in fluorescence gray image

    Fig. 6. Bright field and fluorescence images of cells of Scenedesmus sp., Selenastrum capricornutum and Nostoc sp. (a) Bright images; (b) fluorescence images; (c) binarization images of low frequency component of three-level wavelet decomposition in bright field S channel; (d) binarization images of low frequency component of three-level wavelet decomposition in fluorescence gray image

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    Fusion of bright field and fluorescence images

    Fig. 7. Fusion of bright field and fluorescence images

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    • Table 1. Comparison of bright field and fluorescence images registration methods

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      Table 1. Comparison of bright field and fluorescence images registration methods

      SampleMethodMismatch rate /%Time /sNMI
      Scenedesmus  sp.PSO0138.690.608
      Powell50.910.280.302
      GA6.134.210.524
      Ours010.430.673
      Selenastrum capricornutumPSO17.5476.930.412
      Powell87.511.210.069
      GA35.688.930.325
      Ours9.427.980.495
      Nostoc sp. PSO7.2254.220.525
      Powell73.8 6.140.237
      GA16.750.760.451
      Ours6.517.020.631
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    Renqing Jia, Gaofang Yin, Nanjing Zhao, Min Xu, Xiang Hu, Peng Huang, Tianhong Liang, Qianfeng He, Xiaowei Chen, Tingting Gan, Xiaoling Zhang, Mingjun Ma. Registration Method of Microscopic Bright Field and Fluorescence Synchronous Measurement Images of Phytoplankton Cells[J]. Chinese Journal of Lasers, 2022, 49(24): 2407202

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    Paper Information

    Category: Biomedical Optical Imaging

    Received: Jul. 15, 2022

    Accepted: Aug. 8, 2022

    Published Online: Nov. 15, 2022

    The Author Email: Yin Gaofang (gfyin@aiofm.ac.cn), Zhao Nanjing (njzhao@aiofm.ac.cn)

    DOI:10.3788/CJL202249.2407202

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology