Fig. 1. Open-top two-photon light sheet microscope. (a) Experimental setup; (b) optical path diagram

Fig. 2. Basic principle of Bessel beam modulated by axicon. (a) Schematic diagram of Bessel beam modulated by axicon; (b) cross-section of a Bessel beam

Fig. 3. Curves of effective depth of field L varying with incident beam radius ω₀at different axicon angles

Fig. 4. Simulation results of Gaussian, planar Airy, and Bessel light sheet under two-photon excitation. (a) PSF; (b) MTF; (c) image simulation results; (d) simulation of imaging deconvolution results

Fig. 5. Fluorescence microsphere imaging results of Gaussian light sheet and Bessel light sheet under two-photon excitation. (a) Fluorescence microsphere imaging results under Gaussian light sheet; (b) fluorescence microsphere imaging results under Bessel light sheet; (c) statistical fitting results of fluorescent microsphere FWHM; (d) statistical fitting results of fluorescence microsphere intensity

Fig. 6. Three-dimensional imaging results of different regions of mice kidney. (a) Three-dimensional images of kidneys in region I of Sham group mice; (b) three-dimensional images of kidneys in region II of Sham group mice; (c) three-dimensional images of kidneys in region I of CP group mice; (d) three-dimensional images of kidneys in region II of CP group mice