Chinese Journal of Lasers, Volume. 45, Issue 12, 1207001(2018)

Large Field-of-View and Deep Tissue Optical Micro-Imaging Based on Parallel Wavefront Correction Algorithm

Qi Zhao1、*, Xin Shi1, Wei Gong2, Lejia Hu1, Yao Zheng1, Xinpei Zhu2, and Ke Si1,2、*
Author Affiliations
  • 1 State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China
  • 2 Center for Neuroscience, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China
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    As for deep tissues, the field of view of a single correction in the widely used adaptive optics is limited and the refresh rate of a spatial light modulator or a deformable mirror is also limited. Therefore it is difficult for them to satisfy the requirement of large field-of-view (FOV) rapid correction of wavefront distortion and thus that of high-speed imaging. A parallel wavefront correction method is proposed based on the conjugate adaptive optical correction system and the coherent optical adaptive technique. In this method, without increasing the number of refresh times of spatial light modulator, the large FOV of one-time correction can be realized by means of the parallel measurement of wavefront distortion of multiple guide stars, which provides a feasible reference solution for the high-speed and high-resolution imaging of deep tissues. The simulation results show that when 9 guiding stars are used, the effective FOV of a single correction by the proposed method is about 4.7 times than that of the conventional method for a thin scattering medium composed of 5 layers of random phase masks, and 4.6 times than that of the conventional method for 120-μm thick mouse brain tissue. Moreover, the proposed method can further improve the FOV of one-time correction by increasing the number of guide stars while the correction time does not significantly increase, which has broad application prospect in the large FOV imaging of in vivo biological tissues.

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    Qi Zhao, Xin Shi, Wei Gong, Lejia Hu, Yao Zheng, Xinpei Zhu, Ke Si. Large Field-of-View and Deep Tissue Optical Micro-Imaging Based on Parallel Wavefront Correction Algorithm[J]. Chinese Journal of Lasers, 2018, 45(12): 1207001

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Jul. 4, 2018

    Accepted: Aug. 5, 2018

    Published Online: May. 9, 2019

    The Author Email:

    DOI:10.3788/CJL201845.1207001

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