Fig. 1. Schematic of the hyperspectral imaging system

Fig. 2. Flowchart of the clinical data collection process

Fig. 3. Process for calculating melanin content index and StO₂ index in vulvar skin tissue. (a)-(c) Hyperspectral images of the vulva, PTFE board, and dark background; (d) relative reflectance image of the vulva at 550 nm wavelength; (e) the raw intensity curve and the relative reflectance curve retrieved from the dot area in Fig.3 (d); (f) corresponding melanin content index; (g) corresponding StO₂ index

Fig. 4. Hyperspectral analysis results of two VLS patients. (a) Colposcopy images of the vulva, circles are the suspicious disease areas identified by the clinician; (b) corresponding relative reflectance images at 550-nm wavelength; (c) absorption spectra between normal tissues and VLS tissues; (d) melanin content index maps; (e) StO₂ index maps

Fig. 5. Statistical comparison of melanin content index and StO₂ index of 40 VLS patients