Fig. 1. Optical layout of the dual-color light-sheet flow imaging system

Fig. 2. Images and analysis before and after dual-channel registration. (a) 488 nm channel fluorescent microsphere image; (b) 561 nm channel fluorescent microsphere image; (c) dual-channel fusion image before registration; (d) dual-channel merged image after registration; (e) magnified image of the yellow box area in Fig.2(c); (f) magnified image of the yellow box area in Fig.2(d); (g) fluorescence intensity distribution and fitting curve at the straight line in Fig.2(e) and Fig.2(f); (h) distribution histogram of the distance between fluorescent microspheres before and after registration in the X direction; (i) distribution histogram of the distance between fluorescent microspheres before and after registration in the Y direction

Fig. 3. 3D images of vasculature structure and erythrocytes distribution of zebrafish sample and its 2D projection in 9 dpf. (a) Dual-channel 3D fusion image of zebrafish; (b) 2D projection of zebrafish vasculature structure image under 488 nm channel; (c) 2D projection of zebrafish erythrocytes distribution image under 561 nm channel; (d) dual-channel 2D fusion projection of zebrafish; (e) detail of dual-channel 3D fusion image of zebrafish before registration; (f) detail of dual-channel 3D fusion image of zebrafish after registration

Fig. 4. 3D merged images of vasculature structure and erythrocytes distribution of 4 dpf zebrafish and 9 dpf zebrafish. (a) Dual-channel 3D image of 4 dpf zebrafish; (b) dual-channel 3D image of 9 dpf zebrafish; (c) enlarged view of the marked area of the image in Fig.4(a); (d) enlarged view of the marked area of the image in Fig.4(b); (e) 2D projection of the marked area of the image in Fig.4(a); (f) 2D projection of the marked area of the image in Fig.4(b)