Development of a compact fluorescence point-of-care system (POCS) is challenging. A prototype of compact fluorescence detection system and disposable microfluidic chip reported in this letter used a blue LED, 600-micron fiber, CCD detector, mylar and polymethyl-methacrylate (PMMA), and fluorescein solution as an excitation source, light-guide, detector, microfluidic chip substrates and samples, respectively. The results show the system is able to detect 0.01-1000 micro-g/l fluorescein in tris buffered saline pH 8.0 and gives linear response. This prototype can be used as a platform to develop a simple and compact multichannel-fluorescence point-of-care bioassay system with an inexpensive and disposable microfluidic chip.