Fig. 1. Optical path layout of the RCM system. APD, avalanche photodiode; PBS, polarizing beam splitter; QWP, quarter-wave plate; RCM, reflectance confocal microscope.

Fig. 2. Electrical control and data acquisition diagram of the RCM.

Fig. 3. Control principle diagram in the RCM system.

Fig. 4. FPGA controller realized synchronization of galvanometer scanning, data acquisition, and image formation.

Fig. 5. Demonstration of in vivo video-rate imaging of human skin using the RCM system we designed. Scale bar, 150 μm; frame rate, 11 fps.

Fig. 6. Optical path of the SRS microscope. EOM, electro-optic modulator; HWP, half-wave plate.

Fig. 7. Electrical and data acquisition diagram of the SRS microscope.

Fig. 8. SRS images of live SKOV-3 ovarian cancer cells at 2854cm−1 (left, CH2; lipids) and 2940cm−1 (CH3, proteins). The cell line was purchased from American Type Culture Collection (ATCC) and cultured in McCoy’s 5A Medium (ATCC) with 10% fetal bovine serum (ATCC) for 24 h on a glass coverslip before imaging. The images were acquired with 1024×1024pixels, and the dwell time of each pixel was 10 μs.