WindSTORM PLUS Algorithm with Parallel Computing Optimization

Xiao Wen; Wu Tianqi; Li Renjian; Tang Li; Chen Lingling

doi:10.3788/CJL202047.0607001

Chinese Journal of Lasers, Volume. 47, Issue 6, 607001(2020)

WindSTORM PLUS Algorithm with Parallel Computing Optimization

Xiao Wen^1,2, Wu Tianqi^1,2, Li Renjian^1,2, Tang Li^1,2、*, and Chen Lingling^1,2

¹College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, Guangdong 518060, China

²College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen, Guangdong 518118, China

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Figures & Tables(7)

Fig. 1. Schematics of architecture. (a) GPU architecture; (b) MATLAB CPU parallel architecture

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Fig. 2. Flow chart of WindSTORM PLUS program design

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Fig. 3. Comparison of processing speed of different algorithms using simulated data sets. (a) Data set with low density; (b) data set with high density

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Fig. 4. Schematic of STORM system

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Fig. 5. Reconstruction results of microtubule stained by Alexa Fluor 647. (a) Wide-field fluorescence image; (b) super-resolution image reconstructed by ThunderSTORM(Phasor); (c) super-resolution image reconstructed by ThunderSTORM(Gauss-WLS); (d) super-resolution image reconstructed by WindSTORM PLUS; (e) normalized intensity fitting of the line of ROI; (f) calculated FRC resolution (R)

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Fig. 6. Super-resolution image of HEK-293 actin. (a) Wide-field fluorescence image; (b) super-resolution image reconstructed using ThunderSTORM (Phasor); (c) super-resolution image reconstructed using ThunderSTORM (Gauss-WLS); (d) super-resolution image reconstructed using WindSTORM PLUS

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Table 1. Accuracy evaluation (F₁ score) of different algorithms using simulated data sets%
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Table 1. Accuracy evaluation (F₁ score) of different algorithms using simulated data sets%
Data set ThunderSTORM(Phasor) ThunderSTORM(Gauss-WLS) WindSTORM PLUS(and WindSTORM)
Low density (0.2 emitter/μm²) 62.6 97.1 94.7
High density (1 emitter/μm²) 81.4 92.9 94.3

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Xiao Wen, Wu Tianqi, Li Renjian, Tang Li, Chen Lingling. WindSTORM PLUS Algorithm with Parallel Computing Optimization[J]. Chinese Journal of Lasers, 2020, 47(6): 607001

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Paper Information

Category: biomedical photonics and laser medicine

Received: Jan. 15, 2020

Accepted: --

Published Online: Jun. 3, 2020

The Author Email: Li Tang (jac.t.27@gmail.com)

DOI:10.3788/CJL202047.0607001

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Accuracy evaluation (F1 score) of different algorithms using simulated data sets%

Table 1. Accuracy evaluation (F1 score) of different algorithms using simulated data sets%

Table 1. Accuracy evaluation (F₁ score) of different algorithms using simulated data sets%

Table 1. Accuracy evaluation (F₁ score) of different algorithms using simulated data sets%