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Laser & Optoelectronics Progress, Volume. 58, Issue 5, 0526001(2021)

Portable Fluorescence Detection System for Measuring Pepsinogen I

Mingming Xu, Wei Shen, Hua Xia*, Yi Wu, Hui Xue, Sujuan Chen, Xiaole Luo, and Jiapeng Gu
Author Affiliations
  • Optical Engineering Center, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei , Anhui 230031, China
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    AbstractGet PDF(in Chinese)
    Figures&Tables (8)
    Equations (2)
    References (20)
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    Figures & Tables(8)
    Structural of fluorescent immune-chromatographic test strip

    Fig. 1. Structural of fluorescent immune-chromatographic test strip

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    Testing system. (a) Schematic of system; (b) field test picture

    Fig. 2. Testing system. (a) Schematic of system; (b) field test picture

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    Fluorescence image collected in darkroom

    Fig. 3. Fluorescence image collected in darkroom

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    Schematic of image processing process

    Fig. 4. Schematic of image processing process

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    Fluorescence images. (a) Original image; (b) segmentation result of global OTSU; (c) segmentation result of local OTSU

    Fig. 5. Fluorescence images. (a) Original image; (b) segmentation result of global OTSU; (c) segmentation result of local OTSU

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    Standard curve fitting

    Fig. 6. Standard curve fitting

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    • Table 1. T/C value under different PGI mass concentration

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      Table 1. T/C value under different PGI mass concentration

      Mass concentration/(μg·mL-1)06.4454.65107.68130.03174.43213.55239.74276.4
      T/C value0.1450.3391.1111.5331.6121.8712.1092.2442.286

    • Table 2. Test results of T/C value under different PGI mass concentration

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      Table 2. Test results of T/C value under different PGI mass concentration

      Testing No.0 μg/mL6.44 μg/mL54.65 μg/mL107.68 μg/mL130.03 μg/mL174.43 μg/mL213.55 μg/mL239.74 μg/mL276.4 μg/mL
      10.1450.3391.1111.5331.6121.8712.1092.2442.286
      20.1440.3271.1911.4761.6821.8222.1752.2172.286
      30.1390.3381.1411.5121.6001.8062.1712.2992.285
      40.1450.3311.1801.5691.6331.8492.1842.1762.403
      50.1440.3631.2221.4461.6321.8332.2052.3702.372
      60.1440.3261.1151.4671.6291.8342.1802.3582.225
      70.1490.3251.0781.5051.6391.8232.1892.2152.446
      80.1450.3441.1241.4961.6551.8362.1412.3182.426
      90.1460.3261.0391.4591.6321.8882.1692.2252.164
      100.1460.3491.1621.5291.6271.8022.1702.2842.225
      Mean value0.14470.33681.13631.49921.63411.83642.16932.27062.3118
      SD0.002500.012450.054810.038190.022340.026940.026800.065360.09543
      CV/%1.733.694.822.551.371.471.242.884.13
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    Mingming Xu, Wei Shen, Hua Xia, Yi Wu, Hui Xue, Sujuan Chen, Xiaole Luo, Jiapeng Gu. Portable Fluorescence Detection System for Measuring Pepsinogen I[J]. Laser & Optoelectronics Progress, 2021, 58(5): 0526001

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    Paper Information

    Category: Physical Optics

    Received: Jun. 3, 2020

    Accepted: Aug. 3, 2020

    Published Online: Apr. 19, 2021

    The Author Email: Hua Xia (huaxia@aiofm.ac.cn)

    DOI:10.3788/LOP202158.0526001

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology