Photonics Research, Volume. 8, Issue 7, 1253(2020)
Portable quantitative phase microscope for material metrology and biological imaging
Fig. 1. (a) Schematic design of the portable QPM system. (b) Software GUI running on the laptop. (c) Actual portable QPM system. (d) Internal components of the portable QPM system.
Fig. 2. (a) Histogram of the OPD values for all the pixels of all the frames. (b) Temporal fluctuation of the frame-averaged OPD value. (c) Histogram of the OPD standard deviation values. (d) OPD map averaged over all the frames.
Fig. 3. (a) Typical SEM image for a portion of the NIST reference material (RM) 8820 sample showing the L500 nm S500 nm and L500 nm S1000 nm lines (figure adapted from Ref. [28]). (b) Phase map measured by the portable QPM system. Inset figure shows the line profile indicated by the white dotted line.
Fig. 4. (a) Design of the gold electrodes in transistor sample. (b) Height map of the sample from the portable QPM system; inset figure shows the line profile along the white dotted line in (b). (c) AFM image of the sample (Software: NanoScope Analysis 1.5); inset figure shows the line profile along the white dotted line in (c). (d) Height histogram of the Au structure area and the bottom area selected in (b). (e) Height histogram of the Au structure area and the bottom area selected in (c).
Fig. 5. (a) Height map of 5 μm beads. (b) Line profile along the yellow arrow direction for the bead indicated in (a).
Fig. 6. (a) Height map for a selected region in the NIST reference material (RM) 8820 sample. (b) Zoom-in height map for the selected area in (a). (c) SEM image of the selected area (figure adapted from Ref. [28]). (d) Histogram of the topography map in (b).
Fig. 7. (a) Raw interferogram of the RBCs with a zoom-in showing the fringe contrast and bending. (b) Retrieved height map of the RBCs in (a). (c) Snapshot of the membrane height fluctuation in
Fig. 8. (a) Actual microfluidic device and the geometry of the channels. (b) Representative phase image showing the imaging field of view of the microfluidic channel. (c) Histogram of the dry mass for all the cells captured in the microfluidic channel. (d) Phase maps of a few selected myoblasts captured in the microfluidic channel.
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Mengxuan Niu, Gang Luo, Xin Shu, Fuyang Qu, Shuang Zhou, Yi-Ping Ho, Ni Zhao, Renjie Zhou, "Portable quantitative phase microscope for material metrology and biological imaging," Photonics Res. 8, 1253 (2020)
Category: Imaging Systems, Microscopy, and Displays
Received: Apr. 24, 2020
Accepted: Jun. 4, 2020
Published Online: Jun. 30, 2020
The Author Email: Renjie Zhou (rjzhou@cuhk.edu.hk)