The thick nanostructured Al2O3-13%TiO2(mass fraction) ceramic coating is prepared by squash presetting type laser multi-layer cladding. The microstructure and bonding strength of coating are investigated, and the effect of coating thickness on bonding strength is analyzed. The results show that the laser-clad ceramic coating has no obvious interface between the layers but dense, continuous structure, and there is almost no pore and large penetrating crack. The coating is composed of fully melted regions (fine equiaxed grains) and partially melted regions (remained nanoparticles). In addition, most cracks in the coating are concentrated on the partially melted region. Due to multiple heating by laser beam, the grain size of lower layer is bigger than that of upper layer. The bonding strength of coating decreases with the increase of coating thickness, and the trend is fast at first and then slow. The bonding strength of coating with a thickness of 175 μm is beyond 78.6 MPa, while those of 350, 525, 700 μm thickness coatings are 66.3, 47.4, 36.2 MPa, respectively.