Design of Microscopy Imaging System Based on ZYNQ

Cunlong Su; Jing Liu; Runna Liu; Shuwei Yang; Keding Yan; Siyi He; Yang Zhou

doi:10.3788/LOP241644

Laser & Optoelectronics Progress, Volume. 62, Issue 6, 0611003(2025)

Design of Microscopy Imaging System Based on ZYNQ

Cunlong Su^1,2、*, Jing Liu³, Runna Liu³, Shuwei Yang¹, Keding Yan², Siyi He², and Yang Zhou²

Author Affiliations

¹School of Intelligent Science and Engineering, Xi'an Peihua University, Xi'an 710125, Shaanxi , China

²School of Electronic and Information Engineering, Xi'an Technological University, Xi'an 710021, Shaanxi , China

³School of Medicine, Xi'an International University, Xi'an 710077, Shaanxi , China

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Figures & Tables(11)

Fig. 1. Component block diagram of small microimaging system

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Fig. 2. Hardware composition diagram of small microscopic imaging system. (a) Imaging optical path diagram; (b) mechanical structure design diagram; (c) LED spotlight; (d) homogeniser; (e) physical diagram of achromatic objective lens; (f) manual slide; (g) physical diagram of OV5640 camera; (h) ZYNQ main control

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Fig. 3. Block diagram of acquisition system architecture

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Fig. 4. Top-level design of image acquisition system

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Fig. 5. Flowchart of image acquisition system software

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Fig. 6. Software design diagram for small microscopic imaging system. (a) Overall flow chart of microscopic image acquisition software; (b) graphical user interface of microscopic image acquisition software

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Fig. 7. System hardware and software joint diagram

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Fig. 8. System resolution test. (a) Physical image of USAF-1951 resolution board; (b) image of resolution board captured under 10× objective lens

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Fig. 9. Bright-field microscopic images captured under 10× objective lens. (a) Human blood cell; (b) longitudinal section of myocardium; (c) transverse section of spinal cord; (d) transverse section of Cycas ferruginea leaf; (e) slice of fish skin; (f) corn starch

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Table 1. Parameter of flat field achromatic objective
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Table 1. Parameter of flat field achromatic objective
Magnifying power Numerical aperture Working distance /mm Optical resolution /nm Ideal pixel size /μm
10× 0.25 2.04 976‒1854 4.88‒9.27
20× 0.40 0.65 610‒1159 6.10‒11.59
40× 0.65 0.56 375‒713 7.50‒14.26

Table 2. USAF-1951 resolution test table

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Table 2. USAF-1951 resolution test table

Element	0	1	2	3	4	5	6	7
1	500.00	250.00	125.00	62.50	31.25	15.63	7.81	3.91
2	445.45	222.72	111.36	55.68	27.84	13.92	6.96	3.48
3	396.85	198.43	99.21	49.61	24.80	12.40	6.20	3.10
4	353.55	176.78	88.39	44.19	22.10	11.05	5.52	2.76
5	314.98	157.49	78.75	39.37	19.69	9.84	4.92	2.46
6	280.62	140.31	70.15	35.08	17.54	8.77	4.38	2.19

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Cunlong Su, Jing Liu, Runna Liu, Shuwei Yang, Keding Yan, Siyi He, Yang Zhou. Design of Microscopy Imaging System Based on ZYNQ[J]. Laser & Optoelectronics Progress, 2025, 62(6): 0611003

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