A preparation experiment of genechip microarrays was carried out by the microfluid digitalization, in which the pulse is basis forms of the microfluidic flows and the microfluidic flows are driven by the pulsed local inertia force in micro channels. Based on the technology, an experimental system to prepare genechip microarrays was built to study the effect the nozzle inside falloff angle 2θ, nozzle inner diameter d, voltage amplitude U, and the driving frequency f on the droplet diameter and pulse potting stability. The experimental scheme for preparing the microarrays was proposed and then the moderate microarray with the droplet average diameter of 102.2 μm and the density of microarray of 4 000 spot/cm2 were manufactured by using 3×SSC citrate buffer as spotting solution. The results in this paper can provide a experimental basis for establishing a high-throughput microarray pulse spotting technology.