A Straight-Axis Multi-Channel Optical Detection System for Nucleic Acid On-Site Detection

Bangchao Xi; Jiayu Yang; Shaolei Huang; Haozheng Dai; Juntian Zeng; Dongxu Zhang

doi:10.3788/LOP222231

Laser & Optoelectronics Progress, Volume. 60, Issue 17, 1722001(2023)

A Straight-Axis Multi-Channel Optical Detection System for Nucleic Acid On-Site Detection

Bangchao Xi^1,2,3,4, Jiayu Yang^1,2,3,4, Shaolei Huang^1,2,3,4, Haozheng Dai^1,2,3,4, Juntian Zeng^1,2,3,4, and Dongxu Zhang^1,2,3,4、*

Author Affiliations

¹School of Public Health, Xiamen University, Xiamen 361102, Fujian , China

²State Key Laboratory of Molecular Vaccinology and Molecular Diagnostic(Xiamen University), Xiamen 361102, Fujian , China

³National Institute of Diagnostics and Vaccine Development in Infection Diseases(Xiamen University), Xiamen 361102, Fujian , China

⁴State Drug Administration Key Laboratory of Infectious Disease Detection Technology Research and Evaluation, Xiamen 361102, Fujian , China

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Figures & Tables(13)

Fig. 1. Schematic diagram of the orthogonal optical path structure

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Fig. 2. Schematic diagram of light passing through optical components

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Fig. 3. Schematic diagram of multi-channel integration and switching device

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Fig. 4. Rapid amplification supporting device. (a) Microfluidic chip; (b) amplification heating light path; (c) optical fiber with linear spot; (d) physical map of multi-channel integration and switching device

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Fig. 5. Block diagram of the hardware structure of the detection system

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Fig. 6. Light Tools simulation. (a) Optical path structure; (b) spot size

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Fig. 7. Test environment and conditions. (a) Fully automatic nucleic acid detection device; (b) internal structure diagram

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Fig. 8. Resolution comparison between the straight-axis multi-channel optical detection systems and commercial PCR instruments.(a) FAM; (b) HEX; (c) ROX

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Fig. 9. Channel crosstalk data histogram

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Fig. 10. Detection curves of straight-axis multi-channel optical detection systems. (a) Random result 1; (b) random result 2; (c) random result 3; (d) random result 4

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Table 1. Parameters of LED

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Table 1. Parameters of LED

LED	Center wavelength /nm	Half bandwidth /nm	Half-width value /（°）	Company	Fluorescence channel
NSPB510BS	465	20	±15	NICHIA	FAM
NSPG310B	520	35	±15	NICHIA	HEX
HSE575H-L512	575	35	±12	Huashang Optoelectronics	ROX
NSPR310S	635	15	±15	NICHIA	CY5

Table 2. Parameters of optical path distance

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Table 2. Parameters of optical path distance

Fluorescence channel	Distance between LED and excitation lens /mm	Distance between excitation lens and filter/mm	Distance between fiber and emission lens /mm	Distance between emission lens and filter /mm
FAM	7.7	7.3	7.6	4.3
HEX	7.7	7.3	7.6	4.3
ROX	7.1	7.3	7.6	4.3
CY5	7.7	7.3	7.6	4.3

Table 3. Fluorescent dye detection repeatability test results
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Table 3. Fluorescent dye detection repeatability test results
Dye FAM HEX ROX
Concentration High Moderate Low High Moderate Low High Moderate Low
CV /% 0.02 0.10 0.72 0.10 0.18 0.83 0.17 0.54 0.80

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Bangchao Xi, Jiayu Yang, Shaolei Huang, Haozheng Dai, Juntian Zeng, Dongxu Zhang. A Straight-Axis Multi-Channel Optical Detection System for Nucleic Acid On-Site Detection[J]. Laser & Optoelectronics Progress, 2023, 60(17): 1722001

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