In order to observe corneal wound healing process after injury induced by infrared laser at the wavelength of 3.74?μm. The cornea of C57BL/6J mice was damaged by infrared laser at the wavelength of 3.74 μm for exposure duration of 0.8?s and the beam diameter of 2 mm with corneal radiant exposures of 23.2 J/cm2. Gross observation through digital camera, slit lamp microscopy, optical coherence tomography (OCT) and histological evaluation of the cornea were preformed pre-exposure and 3 h, 6 h, 12 h, 1 d, 3 d, 7 d, 14 d, 21 d after injury. It was shown that the corneal lesion was grey-white and could be seen immediately after laser exposure. The anterior surface became unsmooth. The corneal opacity increased gradually and peaked at 1 d and then subsided at 3～7 d, but worsened again at 14～21 d. By slit illumination it could be seen that the corneal lesion involved the whole layer. The corneal thickness increased gradually, then subsided with time, whereas the light scattering increased again at 21 d. OCT showed that the high-reflectance?band?and the corneal thickness from the damaged region increased, and the cornea protruded significantly. At 12 h, the corneal thickness reached maximum, then decreased gradually with time. Histological section showed that at 3 h, the epithelial nuclei were hyperchromatic with pyknosis, the chromatin of epithelial nuclei staining faded and then disappeared at 6～12 h. At 1 d, 1～2 layers of newborn epithelial cells covered the injury area completely. 3～4 layers of epithelial cells could be observed at 3～7 d, and the epithelium returned to normal from 14 d to 21 d. Most chromatin in the stroma cells lost after injury at 3 h, the chromatin of stromal nuclei disappeared and infiltrating cells appeared in the stroma layer at 6～12 h. At 1 d, infiltrating cells increased in the stroma, and the infiltrating cells of the stroma migrated from the deep layer to the shallow layer at 3～7 d. From 14 d to 21 d, the infiltrating cells in the stroma decreased, whereas the stromal fibers were still irregularly arranged. The endothelial layer disappeared completely at 3 h, and a few endothelial cells appeared in the endothelium at 1 d. At 3～7 d, the endothelial cells increased and flattened, and the endothelium returned to normal from 14 d to 21 d. Thus, the cornea lesion induced by 3.74 μm laser at the irradiation dose of 23.2 J/cm2 in mice model involved the whole cornea layer. After injury, the corneal response was enhanced at first, and then?recovered?gradually. The epithelium and endothelium recovered to normal, while the structure of the stroma layer did not return to normal during the observation period. Providing the experimental basis for hazard evaluation of corneal injuryed by laser and treatment.