Acta Laser Biology Sinica, Volume. 31, Issue 2, 134(2022)

Dual-wavelength Decoupling Method of Cell Refractive Index and Thickness Based on Phase Diagram

LIAO Jingrong1, MAO Feifei2, WANG Xinyun2, WANG Ziqi2, XU Yuanyuan2、*, XUE Shuangshuang2, SUN Yujuan2, and WANG Yawei2
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  • 2[in Chinese]
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    References(19)

    [1] [1] WAX A. Phase imaging of mechanical properties of living cells[J]. Optics Optoelectronic Technology, 2020, 18(3): 1672-3392.

    [3] [3] FARKAS D L, TARNOK A, LEARY J F. Proceedings of spie-imaging, manipulation, and analysis of biomolecules, cells, and tissues XVII[C]. San Francisco: Spie, 2019.

    [12] [12] POLLIACK A, FU S M, DOUGLAS S D, et al. Scanning electron microscopy of human lymphocyte-sheep erythrocyte rosettes[J]. Journal of Experimental Medicine, 1974, 140(1): 146-158.

    [13] [13] HENDERSON Е, HAYDON P G, SAKAGUCHI D S. Actin filament dynamics in living glial cells imaged by atomic force microscopy[J]. Science, 1992, 257(5078): 1944-1946.

    [14] [14] MERIAUDEAU F, GOUDONNET J Р, CARVER E, et al. Photon scanning-tunneling microscopy of unstained mammalian cells and chromosomes[J]. Applied Optics, 1998, 37(31): 7276-7288.

    [16] [16] SCHURMANN M, SCHOLZE J, MULLER P, et al. Refractive index measurements of single, spherical cells using digital holographic microscopy[J]. Methods in Cell Biology, 2015, 125: 143-159.

    [17] [17] LUE N, POPESCU G, IKEDA T, et al. Live cell refractometry using microfluidic devices[J]. Optics Letters, 2006, 31(18): 2759-2761.

    [19] [19] CHEN X, KANDEL M E, HU C F, et al. Wolf phase tomography (WPT) of transparent structures using partially coherent illumination[J]. Light, Science & Applications, 2020, 9(1): 1-9.

    [20] [20] DARDIKMAN G, SHAKED N T. Review on methods of solving the refractive index-thickness coupling problem in digital holographic microscopy of biological cells[J]. Optics Communications, 2018, 422(1): 8-16.

    [21] [21] POON T C, DOH K B, SCHILLING B W, et al. Three-dimensional microscopy by optical-scanning holography[J]. Optical Engineering, 1995, 34(5): 1338-1344.

    [22] [22] BELANGER E, LEVESQUE S A, RIOUXPELLERIN E, et al. Measuring absolute cell volume using quantitative-phase digital holographic microscopy and a low-cost, open-source, and 3D-printed flow chamber[J]. Frontiers in Physics, 2019, 7(1): 172.

    [23] [23] RAPPAZ B, CHARRIERE F, DEPEURSINGE C, et al. Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium[J]. Optics Letters, 2008, 33(7): 744-746.

    [24] [24] TURKO N A, SHAKED N T. Erythrocyte volumetric measurements in imaging flow cytometry using simultaneous three-wavelength digital holographic microscopy[J]. Biomedical Optics Express, 2020, 11(11): 6649-6658.

    [25] [25] SO P T C, BEAUREPAIRE E. Proceedings of spie-advanced microscopy techniques II[C]. Munich: Spie, 2011.

    [26] [26] ANTOINE L H, NADINE S N, GENEVIEVE C R, et al. On-chip refractive index cytometry for whole-cell deformability discrimination[J]. Journal Lab on a Chip, 2019, 19(3): 464-474.

    [27] [27] PRZIBILLA S, DARTMANN S, VOLLMER A, et al. Sensing dynamic cytoplasm refractive index changes of adherent cells with quantitative phase microscopy using incorporated microspheres as optical probes[J]. Journal of Biomedical Optics, 2012, 17(9): 097001.

    [28] [28] CHERNOMYRDIN N V, SKOROBOGATIY M, GAVDUSH A A, et al. Quantitative super-resolution solid immersion microscopy via refractive index profile reconstruction[J]. Optica, 2021, 8(11): 1471-1480.

    [29] [29] SHAN M G, KANDEL M E, POPESCU G. Refractive index variance of cells and tissues measured by quantitative phase imaging[J]. Optics Express, 2017, 25(2): 1573-1581.

    [30] [30] WANG Y W, PENG G W, XU Y Y, et al. A method for determining the refractive index of a spheroid cell based on orthogonal phase microscopy[J]. Optics Communications, 2019, 450: 14-21.

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    LIAO Jingrong, MAO Feifei, WANG Xinyun, WANG Ziqi, XU Yuanyuan, XUE Shuangshuang, SUN Yujuan, WANG Yawei. Dual-wavelength Decoupling Method of Cell Refractive Index and Thickness Based on Phase Diagram[J]. Acta Laser Biology Sinica, 2022, 31(2): 134

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    Paper Information

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    Received: Jan. 17, 2022

    Accepted: --

    Published Online: Jul. 25, 2022

    The Author Email: XU Yuanyuan (yuanyuanxulark@126.com)

    DOI:10.3969/j.issn.1007-7146.2022.02.006

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