Fig. 1. Schematics of multi-channel optogenetic system. (a) Design drawing of optical system; (b) 1-to-7 fan-out multimode fiber bundles and fiber incident end image; (c) influence of galvanometer voltage on spot position; (d) test results of stimulation light output frequency and program setting frequency

Fig. 2. Control scheme of multi-channel optogenetic system. (a) Diversity experiment scheme of system; (b) flexible fiber channel selection and sequence setting; (c) software interface; (d) results recorded double-color fluorescence

Fig. 3. Multi-channel fiber power stability and transmittance. (a) Multi-channel fiber end face image and channel No.; (b) 7-channel power stability at 589 nm stimulation light under different power gradients; (c) 7-channel power stability at 473 nm recording light under different power gradients; (d) transmittance of mouse in vitro brain slices with different thicknesses

Fig. 4. Crosstalk test of different fiber channels. (a) Camera base noise; (b) schematics of multi-channel crosstalk test under different modes; (c) test result of two-channel crosstalk during single-channel stimulation; (d) local magnification of Fig.4(c); (e) crosstalk evaluation results of peripheral channels during single-channel stimulation in PL brain region; (f) crosstalk evaluation results of peripheral channels during single-channel stimulation in VTA brain region

Fig. 5. Mice behavior experiment images. (a) Behavior experiment of mice VTA brain region; (b) movement tracks in mice behavior experiment with or without stimulation light; statistical results of (c) travelling distance, (d) speed, (e) angle, and (f) head angle of mice with or without stimulation light