The traditional quality guided phase unwrapping method cannot correctly perform phase unwrapping for multiple objects. To overcome this problem, this study proposes a stereo quality guided phase unwrapping method based on region segmentation. Based on the phase edge of the isolated object, the wrapped phase is divided into several regions, and a stereo matching algorithm establishes the initial point of multi-view binocular phase unwrapping for each isolated region. Furthermore, the quality guided phase unwrapping algorithm realizes the phase unwrapping of multi-view isolated object. A single-frequency fringe structured light three dimensional (3D) measurement based on regional binocular stereo unwrapped phase matching is proposed, which achieves 3D reconstruction of multiple isolated objects under a single-frequency wrapped phase. The experimental results show that the proposed method can achieve a 3D reconstruction of multiple isolated objects in motion. The mean absolute error of diameter for the reconstruction of a standard sphere under four-step phase shifting and single-frame conditions is 0.0135 mm and 0.0347 mm, respectively.