Acta Laser Biology Sinica, Volume. 29, Issue 2, 148(2020)

Analysis of DAPI Staining in Different Tissues of Arabidopsis

YU Jingfang, YIN Runzhu, and ZHOU Jun*
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    References(19)

    [1] [1] DANN O, BERGEN G, DEMANT E, et al. Trypanocide diamidine des 2-Phenyl-benzofurans, 2-Phenyl-indens und 2-Phenyl-indols[J]. Justus Liebigs Annalen der Chemie, 1971, 749(1): 68-89.

    [2] [2] WILSON W D, TANIOUS F A, BARTON H J, et al. Binding of 4’6-diamidino-2-phenylindole (DAPI) to GC and mixed sequences in DNA:intercalation of a classical groove-binding molecule[J]. Journal of the American Chemical Society, 1989, 111(13): 5008-5010.

    [3] [3] TANIOUS F A, VEAL J M, BUCZAK H, et al. DAPI (4’, 6-diamidino-2-phenylindole) binds differently to DNA and RNA:minor-groove binding at AT sites and intercalation at AU sites[J]. Biochemistry, 1992, 31(12): 3103-3112.

    [4] [4] ROSS J, BOON P I, SHARMA R, et al. Variations in the fluorescence intensity of intact DAPI-stained bacteria and their implications for rapid bacterial quantification[J]. Letters in Applied Microbiology, 1996, 22(4): 283-287.

    [5] [5] BUXA S V, PAGLIARI L, MUSETTI R. Epifluorescence microscopy imaging of phytoplasmas in embedded leaf tissues using DAPI and SYTO13 fluorochromes[J]. Microscopie, 2016, 25(1): 49-56.

    [6] [6] GNTHER S, TRUTNAU M, KLEINSTEUBER S, et al. Dynamics of polyphosphate-accumulating bacteria in wastewater treatment plant microbial communities detected via DAPI (4’,6-diamidino-2-phenylindole) and tetracycline labeling[J]. Applied and Environmental Microbiology, 2009, 75(7): 2111-2121.

    [7] [7] LIN M S, ALFI O S. Detection of sister chromatid exchanges by 4’,6-diamidino-2-phenylindole fluorescence[J]. Chromosoma, 1976, 57(3): 219-225.

    [8] [8] ESTANDARTE A K, BOTCHWAY S, LYNCH C, et al. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes[J]. Scientific Reports, 2016, 6(31417): 1-12.

    [9] [9] KAPUS'CI'NSKI J. DAPI:a DNA-specific fluorescent probe[J]. Biotechnic Histochemistry, 1995, 70(5): 220-233.

    [10] [10] KAPUS 'CI'NSKI J, SZER W. Interactions of 4’,6-diamidine-2-phenylindole with synthetic polynucleotides[J]. Nucleic Acids Research, 1979, 6(11): 3519-3534.

    [11] [11] LARSEN T A, GOODSELL D S, CASCIO D, et al. The structure of DAPI bound to DNA[J]. Journal of Biomolecular Structure and Dynamics, 1989, 7(3): 477-491.

    [15] [15] ZHOU J, CHENG M, ZENG L, et al. Specific capture of the hydrolysate on magnetic beads for sensitive detecting plant vacuolar processing enzyme activity[J]. Biosensors and Bioelectronics, 2016, 79(1): 881-886.

    [16] [16] ZHOU J, SUN A, XING D. Modulation of cellular redox status by thiamine-activated NADPH oxidase confers Arabidopsis resistance to Sclerotinia sclerotiorum[J]. Journal of Experimental Botany, 2013, 64(11): 3261-3272.

    [17] [17] DIGBY E M, RANA R, NITZ M, et al. DNA directed damage using a brominated DAPI derivative[J]. Chemical Communications, 2019, 55(67): 9971-9974.

    [18] [18] PARK H B, KAMCEV J, ROBESON L M, et al. Maximizing the right stuff:the trade-off between membrane permeability and selectivity[J]. Science, 2017, 356(6343): 1-10.

    [19] [19] OVER B, MATSSON P, TYRCHAN C, et al. Structural and conformational determinants of macrocycle cell permeability[J]. Nature Chemical Biology, 2016, 12(12): 1065-1074.

    [20] [20] GRAY M W. Mitochondrial genome diversity and the evolution of mitochondrial DNA[J]. Canadian Journal of Biochemistry, 1982, 60(3): 157-171.

    [21] [21] ZENG L, WANG Y, ZHOU J. Spectral analysis on origination of the bands at 437 nm and 475.5 nm of chlorophyll fluorescence excitation spectrum in Arabidopsis chloroplasts[J]. Luminescence, 2016, 31(3): 769-774.

    CLP Journals

    [1] SHI Yalei, YU Jingfang, WU Ke, ZHOU Jun. Genome-wide Fine Insertion/Deletion of Gene Markers in Arabidopsis thaliana[J]. Acta Laser Biology Sinica, 2020, 29(6): 538

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    YU Jingfang, YIN Runzhu, ZHOU Jun. Analysis of DAPI Staining in Different Tissues of Arabidopsis[J]. Acta Laser Biology Sinica, 2020, 29(2): 148

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    Paper Information

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    Received: Nov. 15, 2019

    Accepted: --

    Published Online: Jan. 27, 2021

    The Author Email: Jun ZHOU (zhoujun@scnu.edu.cn)

    DOI:10.3969/j.issn.1007-7146.2020.02.008

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