[3] [3] Chi Z, Xu Q, Zhu L. A Review of Recent Advances in Robotic Cell Microinjection［J］. IEEE Access,2020,8:8520-32.

[4] [4] Sha X, Hui S, Yuliang Z, et al. A review on microscopic visual servoing for micromanipulation systems: applications in micromanufacturing, biological injection, and nanosensor assembly［J］. Micromachines,2019,10:843.

[5] [5] Chow Y T, Chen S, Liu C, et al. A high-throughput automated microinjection system for human cells with small size［J］. IEEE/ASME Transactions on Mechatronics,2016,21(2):838-50.

[7] [7] Liu J, Zhang Z, Wang X, et al. Automated robotic measurement of 3-D cell morphologies［J］. IEEE Robotics and Automation Letters,2017,2(2):499-505.

[8] [8] Zhang L, Ye Y, Dhar R, et al. Estimating dynamic cellular morphological properties via the combination of the RTCA system and a Hough-Transform-Based algorithm［J］. Cells,2019,8(10):1287.

[12] [12] Frangi A F, Niessen W J, Vincken K L, et al. Muliscale vessel enhancement filtering［C］∥Medical Image Computing and Computer-Assisted Intervention-MICCAI'98, First International Conference.Cambridge,MA,USA:IEEE1998:130-137.

[13] [13] Osher S, Sethian J. Fronts Propagating with curvature dependent speed algorithms based on Hamilton-jacobi［J］. Journal of Computational Physics,1988,79:12-49.

[14] [14] Li C, Xu C, Gui C, et al. Distance regularized level set evolution and its application to image segmentation［J］. IEEE Transactions on Image Processing,2010,19(12):3243-54.

[16] [16] Redmon J, Farhadi A. YOLOv3: An incremental improvement［DB］.https:∥arxiv.org/abs/1804.02767,2018.

[17] [17] Lin T Y , Goyal P , Girshick R , et al. Focal Loss for Dense Object Detection［C］∥2017 IEEE International Conference on Computer Vision(ICCV),2017:2999-3007.

[18] [18] Chan T, Vese L A. Active contour without edges［J］. IEEE Trans Image Process,2001,10:266-77.

[19] [19] Li B N, Chui C K, Chang S, et al. Integrating spatial fuzzy clustering with level set methods for automated medical image segmentation［J］. Computers in Biology and Medicine,2011,41(1):1-10.