Automatic microcell injection can overcome the shortcomings of long manual injection and easy fatigue, in order to solve the problem of cell and pinpoint location in automatic intracytoplasmic sperm injection. Based on the method of combining YOLOv3 neural network and level set, the egg cell and the injection needle are divided, and then the needle tip of the injection needle is located. The comparative experiment proved that the method can solve the problem that the traditional level set is difficult to segment cells and injection needles, and completely segment the deformed cells during microinjection as well as locate the needle tip in the cells, and the error is smaller than other algorithms.