Design of Imaging Detection System for Fluorescent Immune-Chromatographic Test Strip

Fig. 4. Segmentation result of fluorescent immune-chromatographic test strip. (a) Original image of fluorescent test strip; (b) binary image after segmentation

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Fig. 5. Image of fluorescent immune-chromatographic test strip with noise

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Fig. 6. Effect images of fluorescent area positioning.(a) Before positioning by algorithm; (b) after positioning by algorithm

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Fig. 7. Picture and testing result of ESEQuant fluorescence analyzer. (a) Picture; (b) testing result

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Fig. 8. Number of per column target points in binary image after segmentation

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Fig. 9. Differences between calculated results using different extended parameters and ESEQuant results

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Fig. 10. Standard fitting curves of concentrations

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Table 1. Evaluation results of segmentation performance
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Table 1. Evaluation results of segmentation performance
Fluorescenceconcentration /(mg·L^-1) U_M C_R
3.125 0.9925 0.4244
12.5 0.9972 0.5342
25 0.9975 0.6153
50 0.9970 0.6498
100 0.9956 0.6575

Table 2. Eigenvalues of test strip with different concentrations tested by ESEQuant and our instrument
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Table 2. Eigenvalues of test strip with different concentrations tested by ESEQuant and our instrument
Fluorescenceconcentration /(mg·L^-1) Our instrument ESEQuant
0 0.2266 0.1338
3.125 0.3614 0.2516
12.5 0.6461 0.5287
25 1.0122 1.0018
50 1.7450 1.6942
100 3.2792 3.4854

Table 3. Fitting results of concentration standard curves
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Table 3. Fitting results of concentration standard curves
Parameter Fitting result
ESEQuant Our instrument
A₁ 0.1562 0.2487
A₂ 430359 7929060
x₀ 7707080 280011000
p 1.0464 0.9956
R² 0.99689 0.99944

Table 4. Repetitive test results of eigenvalues of test strip with different concentrations

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Table 4. Repetitive test results of eigenvalues of test strip with different concentrations

Testing number	Fluorescence concentration /(mg·L^-1)
Testing number	3.125	12.5	25	50	100
1	0.3050	0.6058	1.1549	1.6568	4.6381
2	0.3063	0.6363	1.1955	1.6074	4.5679
3	0.3104	0.5888	1.1078	1.6000	4.5489
4	0.3204	0.6386	1.0873	1.6174	4.5489
5	0.3201	0.6000	1.1103	1.6219	4.5706
6	0.3235	0.5948	1.1030	1.6100	4.5706
7	0.3205	0.5794	1.1372	1.6184	4.5945
8	0.3205	0.5871	1.0828	1.6284	4.5165
9	0.3209	0.6376	1.1568	1.6007	4.5717
10	0.3196	0.6247	1.1421	1.6478	4.6115
Mean value	0.3180	0.6093	1.1278	1.6209	4.5739
S_D	0.0057	0.023	0.0356	0.0190	0.0343
C_v /%	1.79	3.77	3.16	1.17	0.75

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Xu Xiaohan, Xia Guo, Jing Shiqun, Wu Su, Wang Guodong. Design of Imaging Detection System for Fluorescent Immune-Chromatographic Test Strip[J]. Chinese Journal of Lasers, 2018, 45(4): 407005

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Paper Information

Category: biomedical photonics and laser medicine

Received: Jun. 27, 2017

Accepted: --

Published Online: Apr. 13, 2018

The Author Email: Guo Xia (xiaguo@hfut.edu.cn)

DOI:10.3788/CJL201845.0407005

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Evaluation results of segmentation performance

Table 1. Evaluation results of segmentation performance

Table 2. Eigenvalues of test strip with different concentrations tested by ESEQuant and our instrument

Table 2. Eigenvalues of test strip with different concentrations tested by ESEQuant and our instrument

Table 3. Fitting results of concentration standard curves

Table 3. Fitting results of concentration standard curves

Table 4. Repetitive test results of eigenvalues of test strip with different concentrations

Table 4. Repetitive test results of eigenvalues of test strip with different concentrations