Method for Determining Characteristic Time of J and I Points of OJIP Fluorescence Kinetic Curve

Zhichao Ding; Gaofang Yin; Nanjing Zhao; Tingting Gan; Peilong Qi; Mengyuan Gu; Min Chen; Lu Wang; Ming Dong; Peng Huang; Mingjun Ma; Li Fang; Ruifang Yang

doi:10.3788/AOS202242.0826001

Acta Optica Sinica, Volume. 42, Issue 8, 0826001(2022)

Method for Determining Characteristic Time of J and I Points of OJIP Fluorescence Kinetic Curve

Zhichao Ding^1,2,3, Gaofang Yin^1,3、*, Nanjing Zhao^1,3, Tingting Gan^1,3, Peilong Qi^1,2,3, Mengyuan Gu^1,2,3, Min Chen^1,2,3, Lu Wang^1,2,3, Ming Dong^1,2,3, Peng Huang⁴, Mingjun Ma^1,3, Li Fang^1,3, and Ruifang Yang^1,3

Author Affiliations

¹Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China

²University of Science and Technology of China, Hefei, Anhui 230026, China

³Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China

⁴School of Biological Food and Environment, Hefei University, Hefei, Anhui 230601, China

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Figures & Tables(10)

Fig. 1. Measurement system of algae OJIP fluorescence kinetics

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Fig. 2. OJIP curve of Chlorella vulgaris

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Fig. 3. Fluorescence kinetics of Chlorella vulgaris and calculation results of J, I characteristic time. (a) Exponential function analysis method; (b) curvature analysis method

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Fig. 4. Fluorescence kinetics of Chlorella pyrenoidosa and calculation results of J, I characteristic time. (a) Exponential function analysis method; (b) curvature analysis method

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Fig. 5. Fluorescence kinetics of Scenedesmus dimorpha and calculation results of J, I characteristic time. (a) Exponential function analysis method; (b) curvature analysis method

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Fig. 6. Fluorescence kinetics of Cylindrotheca closterium and calculation results of J, I characteristic time. (a) Exponential function analysis method; (b) curvature analysis method

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Fig. 7. OJIP curve of Chlorella vulgaris under DCUM stress

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Fig. 8. V_J varying with DCMU concentration

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Table 1. Results of characteristic time of J, I points for four kinds of algae calculated by exponential function analysis method

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Table 1. Results of characteristic time of J, I points for four kinds of algae calculated by exponential function analysis method

Alga	Average of T_J /ms	RSD of T_J /%	Average of T_I /ms	RSD of T_I /%
Chlorella pyrenoidosa	1.52	1.8	27.15	8.9
Chlorella vulgaris	1.33	1.4	29.90	0.1
Cylindrotheca closterium	1.04	2.2	15.28	4.1
Scenedesmus dimorpha	2.22	8.5	28.80	5.2

Table 2. Change of characteristic time of J point under DCMU stressunit: ms
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Table 2. Change of characteristic time of J point under DCMU stressunit: ms
Concentration of DCMU 0 10 μg·L^-1 20 μg·L^-1 40 μg·L^-1
Curvature analysis 1.80 2.82 1.32 1.22
Exponential function analysis 1.45 1.25 1.18 1.10

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Zhichao Ding, Gaofang Yin, Nanjing Zhao, Tingting Gan, Peilong Qi, Mengyuan Gu, Min Chen, Lu Wang, Ming Dong, Peng Huang, Mingjun Ma, Li Fang, Ruifang Yang. Method for Determining Characteristic Time of J and I Points of OJIP Fluorescence Kinetic Curve[J]. Acta Optica Sinica, 2022, 42(8): 0826001

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