Quantitative Detection Method of Fluorescence Immunochromatography Based on Microscopic Digital Image

Li Tingting; Feng Dawei; Li Qi; Xiang Yang; Jia Qiang; Lu Yutong; Zhao Yang

doi:10.3788/CJL202047.1107001

Chinese Journal of Lasers, Volume. 47, Issue 11, 1107001(2020)

Quantitative Detection Method of Fluorescence Immunochromatography Based on Microscopic Digital Image

Li Tingting¹, Feng Dawei^1、*, Li Qi¹, Xiang Yang¹, Jia Qiang², Lu Yutong¹, and Zhao Yang¹

Author Affiliations

¹School of Opto-Electronic Engineering, Changchun University of Science and Technology, Changchun, Jilin 130022, China

²Laboratory of Space Laser Engineering, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China

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Figures & Tables(11)

Fig. 1. Schematic of optical system imaging

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Fig. 2. Schematic of optical structure of detection system

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Fig. 3. Flow chart of detection method

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Fig. 4. Schematics of area array CCD imaging. (a) Schematic of CCD imaging principle; (b) RGB brightness ladder

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Fig. 5. RGB component pixels brightness histogram. (a) R component pixel brightness histogram; (b) G component pixel brightness histogram; (c) B component pixel brightness histogram

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Fig. 6. Quantum response rate curve

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Fig. 7. Principle of fluorescence immunity reaction. (a) Preparation of test solution; (b) structure of test paper

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Fig. 8. Fluorescence signal intensity curves of urine progesterone (P) molecule with different mass concentrations

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Fig. 9. Test results

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Table 1. CCD related parameters

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Table 1. CCD related parameters

Parameter	Content
Highest resolution	2456×2058
Optical size	2/3
Maximum frame rate /(frame·s^-1)	15
Exposure time /μs	30--5000000
Pixel size /(μm×μm)	3.45×3.45
Photosensitive area /(mm×mm)	8.5×7.1
Data bits	8/14

Table 2. Fitting result
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Table 2. Fitting result
Item Our experiment ESE Quant
Linear expression y₁=-986.0339x₁+10.9533 y₂=34.37304x₂+0.6281
Linear fit $R_{1}^{2}$ =0.9986 $R_{2}^{2}$ =0.9963

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Li Tingting, Feng Dawei, Li Qi, Xiang Yang, Jia Qiang, Lu Yutong, Zhao Yang. Quantitative Detection Method of Fluorescence Immunochromatography Based on Microscopic Digital Image[J]. Chinese Journal of Lasers, 2020, 47(11): 1107001

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