Fig. 1. Chemical structure of CHR

Fig. 2. UV-Visible spectra of CHR in the absence and presence of DNA at 25 ℃ at pH 7.40
[CHR]=9.96×10^-6 mol·L^-1, a—i: [DNA]=(0, 0.14, 0.27, 0.41, 0.54, 0.68, 0.81, 0.94, 1.07)×10^-6 mol·L^-1

Fig. 3. RLS spectra of CHR with and without DNA at 25 ℃ at pH 7.40
[CHR]=2.59×10^-6 mol·L^-1, a—k: [DNA]=(0, 0.35, 0.70, 1.05, 3.42, 1.39, 1.73, 2.07, 2.41, 2.75, 3.08)×10^-6 mol·L^-1

Fig. 4. (a) Changes of RLS intensity of CHR-DNA at different temperature; (b) Changes of RLS intensity of CHR-DNA at different pH at 30 ℃ (p<0.05)
[CHR]=2.59×10^-6 mol·L^-1, [DNA]=1.65×10^-6 mol·L^-1

Fig. 5. (a), (b), (c), (d) was respectively effect of different concentration of NaCl, CaCl₂, Vitamin C, SLS on RLS intensity of CHR-DNA (p<0.05)
[CHR]=2.59×10^-6 mol·L^-1, [DNA]=1.65×10^-6 mol·L^-1