Water Purification Technology, Volume. 44, Issue 7, 23(2025)

Development of In-Situ Generation Mechanism of N2O by Bio-Denitrification in Sludge Aggregates Based on Microelectrodes

KONG Haixia
Author Affiliations
  • Xi'an Branch, North China Municipal Engineering Design & Research Institute Co, Ltd, Xi'an 710018, China
  • show less
    References(38)

    [1] [1] DAVIDSON E A, WINIWARTER W. Urgent abatement of industrial sources of nitrous oxide[J]. Nature Climate Change, 2023, 13(7): 599-601.

    [2] [2] XI J, GONG H, GUO R, et al. Characteristics of greenhouse gases emission from wastewater treatment plants operation in China (2009-2016): A case study using operational data integrated method (ODIM)[J]. Journal of Cleaner Production, 2023, 402: 136829. DOI: 10.1016/j.jclepro.2023.136829.

    [3] [3] MUTHURAMAN G, RAMU A G, MCADAM E, et al. Sustainable removal of N2O by mediated electrocatalytic reduction at ambient temperature electro-scrubbing using electrogenerated Ni (I) electron mediator[J]. Journal of Hazardous Materials, 2019, 378: 120765. DOI: 10.1016/j.jhazmat.2019.120765.

    [4] [4] GRUBER W, VILLEZ K, KIPF M, et al. N2O emission in full-scale wastewater treatment: Proposing a refined monitoring strategy[J]. Science of the Total Environment, 2020, 699: 134157. DOI: 10.1016/j.scitotenv.2019.134157.

    [5] [5] KIM S W, MIYAHARA M, FUSHINOBU S, et al. Nitrous oxide emission from nitrifying activated sludge dependent on denitrification by ammonia-oxidizing bacteria[J]. Bioresource Technology, 2010, 101(11): 3958-3963.

    [6] [6] SONG Z, ZHANG X, NGO H H, et al. Zeolite powder based polyurethane sponges as biocarriers in moving bed biofilm reactor for improving nitrogen removal of municipal wastewater[J]. Science of the Total Environment, 2019, 651: 1078-1086. DOI: 10.1016/j.scitotenv.2018.09.173.

    [7] [7] REVSBECH N P, JORGENSEN B B. Advanced in microbial ecology: Microelectrodes: Their use in microbial ecology[M]//MARSHALL K. Berlin: Springer, 1986: 293-352.

    [9] [9] MASSARA T M, MALAMIS S, GUISASOLA A, et al. A review on nitrous oxide (N2O) emissions during biological nutrient removal from municipal wastewater and sludge reject water[J]. Science of the Total Environment, 2017, 596/597: 106-123. DOI: 10.1016/j.scitotenv.2017.03.191.

    [12] [12] HE Q, ZHU Y, FAN L, et al. Effects of C/N ratio on nitrous oxide production from nitrification in a laboratory-scale biological aerated filter reactor[J]. Water Science and Technology, 2017, 75(6): 1270-1280.

    [13] [13] YU C, TU Q, HUANGFU X, et al. Effects of hydraulic retention time on nitrous oxide production rates during nitrification in a laboratory-scale biological aerated filter reactor[J]. Environmental Technology & Innovation, 2021, 21: 101342. DOI: 10.1016/j.eti.2020.101342.

    [14] [14] LAI J S, MI X C. Ordination analysis of ecological data based on Vegan software package[C]//Xiamen: Proceedings of the 9th National Symposium on Biodiversity Conservation and Sustainable Utilization, 2010.

    [17] [17] REVSBECH N P, CHRISTENSEN P B, NIELSEN L P, et al. Denitrification in a trickling filter biofilm studied by a microsensor for oxygen and nitrous oxide[J]. Water Research, 1989, 23(7): 867-871.

    [18] [18] ANDALIB M, TAHER E, DONOHUE J, et al. Correlation between nitrous oxide (N2O) emission and carbon to nitrogen (COD/N) ratio in denitrification process: A mitigation strategy to decrease greenhouse gas emission and cost of operation[J]. Water Science and Technology, 2018, 77(2): 426-438.

    [19] [19] FENG C, LI Z, ZHU Y, et al. Effect of magnetic powder on nitrous oxide emissions from a sequencing batch reactor for treating domestic wastewater at low temperatures[J]. Bioresource Technology, 2020, 315: 123848. DOI: 10.1016/j.biortech.2020.123848.

    [20] [20] L Y T, ZHANG X Y, ZHU C S, et al. Micro-analysis of nitrous oxide accumulation in denitrification under acidic conditions: The role of pH and free nitrous acid[J]. Journal of Water Process Engineering, 2022, 47: 102767. DOI: 10.1016/j.jwpe.2022.102767.

    [22] [22] ZHOU Z, WANG K, QIANG J, et al. Mainstream nitrogen separation and side-stream removal to reduce discharge and footprint of wastewater treatment plants[J]. Water Research, 2021, 188: 116527. DOI: 10.1016/j.watres.2020.116527.

    [23] [23] WU J, WANG X D, GAO Q F, et al. Mathematical simulation and experimental verification of a novel control strategy for a single stage PN/A (partial nitrification/Anammox) SBR (sequencing batch reactor)[J]. Journal of Environmental Chemical Engineering, 2023, 11(1): 109154.

    [24] [24] ZHANG Q Q, LIU N, LIU J Z, et al. Decoding the response of complete autotrophic nitrogen removal over nitrite (CANON) performance and microbial succession to hydrazine and hydroxylamine: Linking performance to mechanism[J]. Bioresource Technology, 2022, 363: 127948. DOI: 10.1016/j.biortech.2022.127948.

    [25] [25] WU P, CHEN J J, GARLAPATI V K, et al. Novel insights into Anammox-based processes: A critical review[J]. Chemical Engineering Journal, 2022, 444: 136534. DOI: 10.1016/j.cej.2022.136534.

    [26] [26] SLIEKERS A. Completely autotrophic nitrogen removal over nitrite in one single reactor[J]. Water Research, 2002, 36(10): 2475-2482.

    [27] [27] KUAI L, VERSTRAETE W. Ammonium removal by the oxygen-limited autotrophic nitrification-denitrification system[J]. Applied and Environmental Microbiology, 1998, 64(11): 4500-4506.

    [28] [28] JU K, WANG L, LV Y T, et al. Nitrous oxide emission in autotrophic partial nitritation system: Macro- and microanalyses[J]. Journal of Bioscience and Bioengineering, 2015, 120(4): 419-425.

    [30] [30] KONG Q, LIANG S, ZHANG J, et al. N2O emission in a partial nitrification system: Dynamic emission characteristics and the ammonium-oxidizing bacteria community[J]. Bioresource Technology, 2013, 127: 400-406. DOI: 10.1016/j.biortech.2012.10.011.

    [31] [31] KINH C T, SUENAGA T, HORI T, et al. Counter-diffusion biofilms have lower N2O emissions than co-diffusion biofilms during simultaneous nitrification and denitrification: Insights from depth-profile analysis[J]. Water Research, 2017, 124: 363-371. DOI: 10.1016/j.watres.2017.07.058.

    [32] [32] KINH C T, RIYA S, HOSOMI M, et al. Identification of hotspots for NO and N2O production and consumption in counter-and co-diffusion biofilms for simultaneous nitrification and denitrification[J]. Bioresource Technology, 2017, 245: 318-324. DOI: 10.1016/j.biortech.2017.08.051.

    [33] [33] OKABE S, OSHIKI M, TAKAHASHI Y, et al. N2O emission from a partial nitrification-anammox process and identification of a key biological process of N2O emission from Anammox granules[J]. Water Research, 2011, 45(19): 6461-6470.

    [34] [34] WANG X X, FANG F, CHEN Y P, et al. N2O micro-profiles in biofilm from a one-stage autotrophic nitrogen removal system by microelectrode[J]. Chemosphere, 2017, 175: 482-489. DOI: 10.1016/j.chemosphere.2017.02.026.

    [35] [35] L Y T, JU K, SUN T, et al. Effect of the dissolved oxygen concentration on the N2O emission from an autotrophic partial nitritation reactor treating high-ammonium wastewater[J]. International Biodeterioration & Biodegradation, 2016, 114: 209-215. DOI: 10.1016/j.ibiod.2016.01.022.

    [36] [36] L Y T, JU K, WANG L, et al. Effect of pH on nitrous oxide production and emissions from a partial nitritation reactor under oxygen-limited conditions[J]. Process Biochemistry, 2016, 51(6): 765-771.

    [37] [37] ALI M, RATHNAYAKE R M L D, ZHANG L, et al. Source identification of nitrous oxide emission pathways from a single-stage nitritation-Anammox granular reactor[J]. Water Research, 2016, 102: 147-157. DOI: 10.1016/j.watres.2016.06.034.

    [39] [39] LI L, LING Y, WANG H, et al. N2O emission in partial nitritation-Anammox process[J]. Chinese Chemical Letters, 2020, 31(1): 28-38.

    [40] [40] BUNGAY H R, WHALEN W J, SANDERS W M. Microprobe techniques for determining diffusivities and respiration rates in microbial slime systems[J]. Biotechnology and Bioengineering, 1969, 11(5): 765-772.

    [41] [41] SONG J, LI Y, KE D, et al. In situ graphene-modified carbon microelectrode array biosensor for biofilm impedance analysis[J]. Electrochimica Acta, 2022, 403: 139570. DOI: 10.1016/j.electacta.2021.139570.

    [44] [44] JAMESON B D, BERG P, GRUNDLE D S, et al. Continental margin sediments underlying the NE Pacific oxygen minimum zone are a source of nitrous oxide to the water column[J]. Limnology and Oceanography Letters, 2021, 6(2): 68-76.

    [45] [45] MEYER R L, ALLEN D E, SCHMIDT S. Nitrification and denitrification as sources of sediment nitrous oxide production: A microsensor approach[J]. Marine Chemistry, 2008, 110(1/2): 68-76. DOI: 10.1.016/j.marchem.2008.02.004.

    [46] [46] WANG J H, CHEN Y P, DONG Y, et al. A new method to measure and model dynamic oxygen microdistributions in moving biofilms[J]. Environmental Pollution, 2017, 229: 199-209. DOI: 10.1016/j.envpol.2017.05.062.

    [48] [48] PLOUG H, JRGENSEN B. A net-jet flow system for mass transfer and microsensor studies of sinking aggregates[J]. Marine Ecology Progress Series, 1999, 176: 279-290. DOI: 10.3354/meps176279.

    [49] [49] NI B J, CHEN Y P, LIU S Y, et al. Modeling a granule-based anaerobic ammonium oxidizing (ANAMMOX) process[J]. Biotechnology and Bioengineering, 2009, 103(3): 490-499.

    Tools

    Get Citation

    Copy Citation Text

    KONG Haixia. Development of In-Situ Generation Mechanism of N2O by Bio-Denitrification in Sludge Aggregates Based on Microelectrodes[J]. Water Purification Technology, 2025, 44(7): 23

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Received: Mar. 21, 2025

    Accepted: Aug. 25, 2025

    Published Online: Aug. 25, 2025

    The Author Email:

    DOI:10.15890/j.cnki.jsjs.2025.07.003

    Topics