Optical Instruments, Volume. 41, Issue 5, 24(2019)

High frame-rate reconstruction algorithm for laser confocal high speed scanning microscopy

Dan SU, Xiaoyun QIN, Wei ZHOU, Xinyue JIA, and Hanming GUO*
Author Affiliations
  • School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
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    Figures & Tables(7)
    The graph of scan path
    The graph of galvanometer deflection angle change with time
    Image segmentation
    Uniform lattice sequence segmentation results
    The continuous frame feature region difference imaging algorithm processing results
    Similarity analysis of the sequence images obtained by the consecutive frame feature region difference imaging algorithm
    • Table 1. Scan system initialization parameters

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      Table 1. Scan system initialization parameters

      参数
      物镜焦距(f)         33.3 mm
      数据采集卡采样频率(Fc)   106 Hz
      共振振镜频率(Fx)       ≈7 931 Hz
      共振振镜最大偏转角(Amx)  0.8°
      检流计振镜变化周期(2T)   6.56×10−3 s
      检流计振镜最大偏转角(Amy) 0.066°
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    Dan SU, Xiaoyun QIN, Wei ZHOU, Xinyue JIA, Hanming GUO. High frame-rate reconstruction algorithm for laser confocal high speed scanning microscopy[J]. Optical Instruments, 2019, 41(5): 24

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    Paper Information

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    Received: Oct. 30, 2018

    Accepted: --

    Published Online: May. 19, 2020

    The Author Email: Hanming GUO (hmguo@usst.edu.cn)

    DOI:10.3969/j.issn.1005-5630.2019.05.004

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