Spectroscopy and Spectral Analysis, Volume. 41, Issue 12, 3822(2021)
Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry
Figures & Tables(10)
Fig. 1. Chromatogram of Se species with IP-RP-HPLC (Inertsil ODS-3)
Fig. 2. Influences of extraction rates of Se species in samples with different proteases (
Fig. 3. Influences of extraction rates of Se species in samples with different dosage of proteases (trypsin, protease XIV)
Fig. 4. Influences of extraction rates of Se species in samples with different extraction means (
Fig. 5. Influences of extraction rates of Se species in samples with different extraction times by water bath (
Fig. 6. Influences of extraction rates of Se species in samples with different extraction reagents
Table 1. Matrix effects of the 5 kinds of Se species in samples
View tableView in ArticleTable 1. Matrix effects of the 5 kinds of Se species in samples
样品 SeCys2
/%MeSeCys
/%Se(Ⅳ)
/%SeMet
/%Se(Ⅵ)
/%猪肉 93.7 92.1 86.4 108 96.2 鸡肉 88.7 91.2 87.4 113 112
Table 2. Standard recovery rates and precisions of 5 kinds of Se species in pork samples (
n =6)View tableView in ArticleTable 2. Standard recovery rates and precisions of 5 kinds of Se species in pork samples (
n =6)项目 添加浓度/
(mg·kg-1)SeCys2
/%MeSeCys
/%Se(Ⅳ)
/%SeMet
/%Se(Ⅵ)
/%0.025 76.8 86.2 84.4 105 89 回收率 0.10 81.6 90.3 88.4 107 90 0.25 80.7 92.8 88.8 109 96 0.025 7.8 4.9 3.4 5.4 5.7 批内精密度 0.10 5.7 3.2 2.9 4.4 4.0 0.25 5.2 2.7 3.1 4.3 3.5 0.025 12.3 6.9 3.5 6.6 6.5 批间精密度 0.10 9.4 6.0 4.7 5.8 7.3 0.25 8.5 5.7 4.3 6.2 7.7
Table 3. Linear range, linear equation, correlation coefficients and detection limits
View tableView in ArticleTable 3. Linear range, linear equation, correlation coefficients and detection limits
硒形态 线性范围/(μg·L-1) 线性方程 相关系数 检出限/(μg·L-1) SeCys2 5~200 Y=3 843.8X-1 785.9 0.999 1 0.89 MeSeCys 5~200 Y=3 965.7X-1 228.2 0.999 4 0.78 Se(Ⅳ) 5~200 Y=4 858.1X-1 765.7 0.999 8 0.55 SeMet 5~200 Y=3 247.1X-1 620.0 0.999 6 0.94 Se(Ⅵ) 5~200 Y=4 019.5X+956.8 0.999 3 0.70
Table 4. Determination of Se species and total Se contents in samples(
n =3)View tableView in ArticleTable 4. Determination of Se species and total Se contents in samples(
n =3)样品 总硒/
(mg·kg-1)提取率/
%SeCys2/
(mg·kg-1)MeSeCys/
(mg·kg-1)Se(Ⅳ)/
(mg·kg-1)SeMet/
(mg·kg-1)Se(Ⅵ)/
(mg·kg-1)猪肉 0.058±0.043 ND ND 0.149±0.038 ND 0.262±0.010 79.0±3.4 鸡肉 0.074±0.038 ND ND 0.104±0.054 ND 0.219±0.012 81.3±4.2
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Yi-hua WEI, Qing-qing HUANG, Jin-yan ZHANG, Su-yan QIU, Tian-hua TU, Lin-feng YUAN, Ting-can DAI, Biao-jin ZHANG, Wei-hong LI, Han YAN. Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry[J]. Spectroscopy and Spectral Analysis, 2021, 41(12): 3822
Paper Information
Category: Research Articles
Received: Nov. 15, 2020
Accepted: --
Published Online: Dec. 17, 2021
The Author Email: Yi-hua WEI (weiyihua08@163.com)
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