Chinese Journal of Lasers, Volume. 45, Issue 3, 307006(2018)

Combination Light-Sheet Illumination with Super-Resolution Three-Dimensional Fluorescence Microimaging

Xie Xinlin1、*, Chen Rong1, Zhao Yuxuan1, and Fei Peng1,2
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  • 1[in Chinese]
  • 2[in Chinese]
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    Figures & Tables(5)
    (a) Nonscanned light sheet schematic diagram; (b) scaned Gaussian light sheet schematic diagram; (c) different forms of light-sheet mode for different incident rays on the real pupil plane of objective
    Schematic of (a) ultramicroscopy, (b) light-sheet imaging plugin, (c) DSLM, and (d) Bessel plane illumination
    Schematic of (a) STED, (b) PALM, (c) SIM and SSIM, and (d) SOFI
    (a1) Schematic of light-sheet and epi-fluorescence imaging; (a2) comparative result of light-sheet and epi-fluorescence imaging combined with 3B super-resolution algorithm; (b1) Bessel plane illumination microscopy based on SIM; (b2) SIM super-resolution images
    (a) Low resolution 3D cell image by Bessel plane illumination; (b) super resolution image by reconstruction of 3B algorithm; (c) high resolution contrast image under 60×
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    Xie Xinlin, Chen Rong, Zhao Yuxuan, Fei Peng. Combination Light-Sheet Illumination with Super-Resolution Three-Dimensional Fluorescence Microimaging[J]. Chinese Journal of Lasers, 2018, 45(3): 307006

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    Paper Information

    Special Issue:

    Received: Sep. 15, 2017

    Accepted: --

    Published Online: Mar. 6, 2018

    The Author Email: Xie Xinlin (xiexinlin@hust.edu.cn)

    DOI:10.3788/CJL201845.0307006

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