With increasing demands for clean production and power grid peak shaving, CO₂ hydrate is regarded as a promising medium for CO₂ and cold energy transportation. The phase transition and formation characteristics of CO₂ hydrate are crucial for the advancement, optimization, and development of cold storage technologies. However, as a cold storage medium, it faces challenges such as stringent formation conditions and low gas absorption capacity. The formation characteristics of CO₂ hydrates containing thermodynamic promoters, tetra-n-butylammonium bromide (TBAB) and cyclopentane (CP), were investigated using a stirred reactor under relatively low pressure of 2 MPa and a subcooling temperature of 9 K. The experimental results indicate that both TBAB and CP can effectively improve the formation conditions of hydrates, with CP demonstrating the most significant enhancement. Among all additive concentrations, 3% (mole fraction) CP (approximately 10% (mass fraction)) exhibited the highest gas consumption of 35.05 mmol/mol, indicating the strongest gas absorption capacity. The addition of low concentrations of L-tryptophan to systems containing 10% (mass fraction) TBAB and 3% (mole fraction) CP significantly increased the CO₂ absorption in the hydrate systems. Specifically, the gas consumption in the TBAB system increased by 22.4% with the addition of 100×10^-6 (mass ratio) L-tryptophan, while the CP system showed a 6% increase in gas consumption with the addition of 1 000×10^-6 (mass ratio) L-tryptophan.