Chinese Optics Letters, Volume. 14, Issue 7, 071702(2016)

Early-photon guided reconstruction method for time-domain fluorescence lifetime tomography

Lin Zhang1, Chuangjian Cai1, Yanlu Lv1, and Jianwen Luo1,2、*
Author Affiliations
  • 1Department of Biomedical Engineering, Tsinghua University, Beijing 100084, China
  • 2Center for Biomedical Imaging Research, Tsinghua University, Beijing 100084, China
  • show less
    Figures & Tables(6)
    Schematic of the measurement system.
    Reconstruction results of (a) and (b) Expt. 1 and (c) and (d) Expt. 2. (a) and (c) denote the inverse lifetime maps obtained by the EPFLT method, while (b) and (d) denote those obtained by the DFLT method. The red and green circles denote the phantom border and the position of fluorescence targets, respectively.
    Reconstruction results of (a) and (b) Expt. 3 and (c) and (d) Expt. 4. (a) and (c) denote the inverse lifetime maps obtained by the EPFLT method, while (b) and (d) denote those obtained by the DFLT method. The red and green circles denote the phantom border and the position of fluorescence targets, respectively.
    Profiles along the dashed-dotted lines in Figs. 2 and 3 for (a) Expt. 1, (b) Expt. 2, (c) Expt. 3, and (d) Expt. 4. The red lines denote the real inverse lifetime profiles, while the blue and green lines denote the inverse lifetime results obtained by the EPFLT and DFLT methods, respectively.
    • Table 1. Comparison of Fluorescence Lifetimes and Relative Errors by the EPFLT and DLFT Methods

      View table
      View in Article

      Table 1. Comparison of Fluorescence Lifetimes and Relative Errors by the EPFLT and DLFT Methods

      Expt.SolventτEP(ns)τD(ns)Real lifetime (ns)REEP (%)RED (%)
      1DMSO0.8741.0680.979.910.1
      DMSO0.9421.0370.972.96.9
      2DMSO0.8270.8430.9714.713.1
      ALC0.6520.6400.625.23.2
      3DMSO1.0221.0190.975.45.0
      DMSO0.9980.9930.972.92.4
      4DMSO0.9021.0670.977.010.0
      ALC0.6720.8530.628.437.5
    • Table 2. Comparisons of Contrasts and Crosstalks

      View table
      View in Article

      Table 2. Comparisons of Contrasts and Crosstalks

      Expt.MethodCNRRD (%)Real RD (%)RC (%)
      1EPFLT7.377.800
      DFLT2.383.0046.6
      2EPFLT6.0026.856.450
      DFLT2.3031.756.4553.8
      3EPFLT4.452.400
      DFLT2.652.0063.5
      4EPFLT4.3934.256.450
      DFLT2.448.556.4577.7
    Tools

    Get Citation

    Copy Citation Text

    Lin Zhang, Chuangjian Cai, Yanlu Lv, Jianwen Luo, "Early-photon guided reconstruction method for time-domain fluorescence lifetime tomography," Chin. Opt. Lett. 14, 071702 (2016)

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category: Medical optics and biotechnology

    Received: Feb. 25, 2016

    Accepted: May. 5, 2016

    Published Online: Aug. 3, 2018

    The Author Email: Jianwen Luo (luo_jianwen@tsinghua.edu.cn)

    DOI:10.3788/COL201614.071702

    Topics